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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_A-D >  ApoB-100_Human

Human ApoB-100 ELISA Kit

Apolipoproteins are carrier proteins that bind lipids to form water-soluble lipoprotein particles that can be carried through blood and lymph. Several different classes and subclasses of apolipoproteins are known. Apolipoprotein B (ApoB) is the primary apolipoprotein in chylomicrons (lipoprotein particles that contain triglycerides, phospholipids, cholesterol, and proteins) and low-density lipoprotein (LDL). It is also known as FLDB and LDLCQ4. High levels of ApoB can lead to plaques that cause atherosclerosis, and ApoB levels can be a better indicator of heart disease risk than total cholesterol or LDL. The APOB transcript is subject to tissue-specific RNA editing, resulting in two major isoforms, ApoB-100 and ApoB-48.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human ApoB-100 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure human ApoB-100 protein in serum, EDTA plasma, or cell culture supernatant.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring human ApoB-100 protein
  • Sensitivity of 0.03 µg/mL

Applications

  • Measuring human ApoB-100 protein in serum, EDTA plasma, or cell culture supernatant

Components

Precoated plate: Anti-Human ApoB-100 Rabbit IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human ApoB-100
(35B1) Mouse IgG MoAb Fab Affinity-Purified
0.4 mL x 1
Standard: Human ApoB-1000.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 0.13–8.4 µg/mL
  • Assay sensitivity: 0.03 µg/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Human ApoB-100; does not cross-react with human ApoB-48
  • Test specimen type: Serum, EDTA plasma, or cell culture supernatant
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27181A Human ApoB-100 Assay Kit - IBL 96-Well $849.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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