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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_A-D >  Angiotensinogen

Angiotensinogen ELISA Kits

Human angiotensinogen (also known as serpin peptidase inhibitor, clade A, member 8) is a 452-amino acid hormone precursor produced by the liver and is implicated in blood pressure control. Urinary excretion of angiotensinogen in a rat hypertension model indicates that this protein is involved in the intrarenal renin-angiotensin system (RAS). In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-I, which is is further cleaved by angiotensin-converting enzyme (ACE) to yield the physiologically active peptide angiotensin-II. Angiotensin-II stimulates aldosterone release, promoting sodium retention in the kidney and an increase in blood pressure.

These ELISA kits are a solid-phase sandwich ELISAs that take advantage of two highly specific antibodies; one antibody is coated onto the ELISA plate and the other is HRP-conjugated.

  • The Human, Mouse, and Rat Total Angiotensinogen Assay Kits are ELISA kits that can be used to assay serum, EDTA-plasma, urine or cell culture supernatant for total human angiotensinogen, mouse angiotensinogen, or rat angiotensinogen, respectively.
  • The Mouse and Rat Intact Angiotensinogen Assay Kits are ELISA kits that can be used to assay serum, EDTA-plasma, urine, or cell culture supernatant for intact mouse or rat angiotensinogen, respectively.

  At-A-Glance   Documents   Images & Data

Features

Cat.# 27412, 27413, 27414, 27743, 27744
  • Capable of measuring total or intact human, mouse or rat angiotensinogen
  • Sensitivity of 0.03 ng/mL (Cat.# 27412, 27413); 0.02 ng/mL (Cat.# 27744); 0.01 ng/mL (Cat.# 27414, 27743)

Applications

  • Measurement of human, mouse or rat angiotensinogen in serum, EDTA-plasma, urine or cell culture media

Components

Precoated plate: Anti-AGT Rabbit IgG
Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP-conjugated
Anti-AGT IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Angiotensinogen0.5 mL x 2 (Cat # 27412);
1.0 mL x 2 (Cat # 27413, 27414, 27743, 27744)
EIA buffer:30 mL x 1
Solution for labeled antibody: 1% BSA,
0.05% Tween 20 in PBS
12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in
phosphate buffer
50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 0.31–20 ng/mL (Cat.# 27412); 0.16–10 ng/mL (Cat.# 27413, 27743); 0.08–5 ng/mL (Cat.# 27414, 27744)
  • Assay sensitivity: 0.03 ng/mL (Cat.# 27412, 27413); 0.02 ng/mL (Cat.# 27744); 0.01 ng/mL (Cat.# 27414, 27743)
  • Specificity: Human, mouse or rat angiotensinogen; shows ≤0.1 cross-reactivity against angiotensin I-IV proteins
  • Species cross-reactivity: n/a
  • Test specimen type: Serum, EDTA-plasma, urine or cell culture media
  • Sample volume per well: 100 µL



 
 
Products
New Offers Cat. # Product Contents Size Price Units Select
  27412A Human Total Angiotensinogen Assay Kit - IBL 96-Well $849.00 *
  27412D Human Angiotensinogen EIA buffer 100 100 mL $172.00
  27413A Mouse Total Angiotensinogen Assay Kit - IBL 96-Well $849.00 *
  27413D Mouse/Rat Angiotensinogen EIA buffer 100 100 mL $172.00
  27414A Rat Total Angiotensinogen Assay Kit - IBL 96-Well $849.00 *
  27414D Rat Total Angiotensinogen EIA Buffer 30 mL $75.00
27743A Mouse Intact Angiotensinogen Assay Kit - IBL 96-Well $924.00
27744A Rat Intact Angiotensinogen Assay Kit - IBL 96-Well $924.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 
 

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