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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_A-D >  Angiotensinogen

Angiotensinogen ELISA Kits

The Human Angiotensinogen ELISA is a solid-phase sandwich ELISA that takes advantage of two highly specific antibodies; one antibody is coated onto the ELISA plate and the other is HRP-conjugated. The Human Angiotensinogen ELISA can be used to measure human, mouse, or rat angiotensinogen in serum, EDTA-plasma, urine or cell culture media.

Human angiotensinogen (also known as serpin peptidase inhibitor, clade A, member 8) is a 452-amino acid hormone precursor produced by the liver and is implicated in blood pressure control. Urinary excretion of angiotensinogen in a rat hypertension model indicates that this protein is involved in the intrarenal renin-angiotensin system (RAS). In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-I, which is is further cleaved by angiotensin-converting enzyme (ACE) to yield the physiologically active peptide angiotensin-II. Angiotensin-II stimulates aldosterone release, promoting sodium retention in the kidney and an increase in blood pressure.

At-A-Glance Documents Images & Data

Features

Cat.# 27412, 27413, 27414
  • Capable of measuring human, mouse or rat angiotensinogen
  • Sensitivity of 0.03 ng/mL (Cat.# 27412, 27413); 0.01 ng/mL (Cat.# 27414)

Applications

  • Measurement of human, mouse or rat angiotensinogen in serum, EDTA-plasma, urine or cell culture media

Components

Precoated plate: Anti-AGT Rabbit IgG
Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP-conjugated
Anti-AGT IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Angiotensinogen0.5 mL x 2 (Cat # 27412);
1.0 mL x 2 (Cat # 27413, 27414)
EIA buffer:30 mL x 1
Solution for labeled antibody: 1% BSA,
0.05% Tween 20 in PBS
12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in
phosphate buffer
50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 0.31–20 ng/mL (Cat.# 27412); 0.16–10 ng/mL (Cat.# 27413); 0.08–5 ng/mL (Cat.# 27414)
  • Assay sensitivity: 0.03 ng/mL (Cat.# 27412, 27413); 0.01 ng/mL (Cat.# 27414)
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Human, mouse or rat angiotensinogen; shows ≤0.1 cross-reactivity against angiotensin I-IV proteins
  • Species cross-reactivity: n/a
  • Test specimen type: Serum, EDTA-plasma, urine or cell culture media
  • Sample volume per well: 100 µL



 
 
Products
Cat. # Product Package Size Price # of Units Select
27412A Human Total Angiotensinogen Assay Kit - IBL 96-Well $849.00 *
27412D Human Angiotensinogen EIA buffer 100 100 mL $172.00
27413A Mouse Total Angiotensinogen Assay Kit - IBL 96-Well $849.00 *
27413D Mouse/Rat Angiotensinogen EIA buffer 100 100 mL $172.00
27414A Rat Total Angiotensinogen Assay Kit - IBL 96-Well $849.00 *
27414D Rat Total Angiotensinogen EIA Buffer 30 mL $75.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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