The Human Angiotensinogen ELISA is a solid-phase sandwich ELISA that takes advantage of two highly specific antibodies; one antibody is coated onto the ELISA plate and the other is HRP-conjugated. The Human Angiotensinogen ELISA can be used to measure human, mouse, or rat angiotensinogen in serum, EDTA-plasma, urine or cell culture media.
Human angiotensinogen (also known as serpin peptidase inhibitor, clade A, member 8) is a 452-amino acid hormone precursor produced by the liver and is implicated in blood pressure control. Urinary excretion of angiotensinogen in a rat hypertension model indicates that this protein is involved in the intrarenal renin-angiotensin system (RAS). In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-I, which is is further cleaved by angiotensin-converting enzyme (ACE) to yield the physiologically active peptide angiotensin-II. Angiotensin-II stimulates aldosterone release, promoting sodium retention in the kidney and an increase in blood pressure.