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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_A-D >  Angiopoietin_Like_3_Mouse

Mouse ANGPTL3 ELISA Kit

Angiopoietin-like protein 3 (ANGPTL3) is a member of the angiopoietin-like protein family that is expressed primarily in the liver. Like angiopoietins, ANGPTL3 has a signal peptide, N-terminal coiled-coil domain, and a C-terminal fibrinogen (FBN)-like domain. The FBN domain in ANGPTL3 interacts with alpha-5 and beta-3 integrins, leading to induction of endothelial cell adhesion and migration. ANGPTL3 may play a role in angiogenesis. In humans, it is positively correlated with high-density lipoprotein (HDL) cholesterol levels. ANGPTL3 is also known as angiopoietin-like 3, angiopoietin-5 (ANGPT5), ANG-5, and FHBL2.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to mouse ANGPTL3 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble mouse ANGPTL3 protein in serum, EDTA plasma, and cell culture supernatants.

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Features

  • Capable of measuring mouse ANGPTL3 protein
  • Sensitivity: 0.04 ng/mL

Applications

  • Measuring mouse ANGPTL3 protein in serum, EDTA plasma, and cell culture supernatants

Components

Precoated plate: Anti-Mouse Angiopoietin-like 3 Rabbit IgG
Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Mouse
Angiopoietin-like 3 Rabbit IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Mouse Angiopoietin-like 30.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution 15 mL x 1
Stop solution:  1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 0.31–20 ng/mL
  • Assay sensitivity: 0.04 ng/mL
  • Assay duration: Approximately 3–3.5 hours
  • Test specimen type: Serum, EDTA plasma, and cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Offers Cat. # Product Contents Size Price Units Select
27410A Mouse angiopoietin-like 3 Assay Kit - IBL 96-Well $606.00 *
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