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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_A-D >  ANGPTL4_Human

Human ANGPTL4 ELISA Kit

Angiopoietin-like protein 4 (ANGPTL4) is a secreted glycoprotein that has strong structural similarity to angiopoietins. Like angiopoietins, ANGPTL4 has a signal peptide, N-terminal coiled-coil domain, and a C-terminal fibrinogen (FBN)-like domain. ANGPTL4 is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. It also functions as an apoptosis survival factor in vascular endothelial cells. ANGPTL4 is thought to have an antimetastatic function in several cancers; it has been shown to inhibit vascular activity as well as tumor cell motility and invasiveness. Reduced expression of the ANGPTL4 gene has been associated with type 2 diabetes. ANGPTL4 is also known as angiopoietin-like 4, angiopoietin-related protein 4 (ARP4), hepatic fibrinogen/angiopoietin-related protein (HFARP1), peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein (PGAR), PPARG angiopoietin related protein, fasting-induced adipose factor (FIAF), NL2, and pp1158.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human ANGPTL4 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human ANGPTL4 protein in serum, EDTA plasma, and cell culture supernatants.*

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Features

  • Capable of measuring human ANGPTL4 protein
  • Sensitivity: 17.53 pg/mL

Applications

  • Measuring human ANGPTL4 protein in serum, EDTA plasma, and cell culture supernatants.*

Components

Precoated plate: Anti-Human ANGPTL4 (K3-21A1A) Mouse IgG
MoAb Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human ANGPTL4
(K2-19A1A) Mouse IgG MoAb Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Human Full-Length ANGPTL40.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 23.44–1500 pg/mL
  • Assay sensitivity: 17.53 pg/mL
  • Assay duration: Approximately 3–3.5 hours
  • Test specimen type: Serum, EDTA plasma, and cell culture supernatants*
  • Specificity: Shows <0.1% cross-reactivity with human ANGPTL2 and ANGPTL3
  • Sample volume per well: 100 µL

*Note: Cell culture media containing FBS or FCS will give high ANGPTL4 readings.


 
Products
Cat. # Product Contents Size Price Units Select
27749A Human ANGPTL4 Assay kit - IBL 96-Well $849.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

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