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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_A-D >  ANGPTL3_Human

Human ANGPTL3 ELISA Kit

Angiopoietin-like protein 3 (ANGPTL3) is a secreted glycoprotein that has strong structural similarity to angiopoietins. Like angiopoietins, ANGPTL3 has a signal peptide, N-terminal coiled-coil domain, and a C-terminal fibrinogen (FBN)-like domain. The FBN domain in ANGPTL3 interacts with alpha-5 and beta-3 integrins, leading to induction of endothelial cell adhesion and migration. ANGPTL3 may play a role in angiogenesis. It is expressed primarily in the liver and positively correlates with high-density lipoprotein cholesterol (HDL-C) levels. Loss-of-function mutations in one copy of the ANGPTL3 gene are associated with lower levels of low-density lipoprotein cholesterol (LDL-C); in both copies, with lower levels of LDL-C, HDL-C, and triglycerides. ANGPTL3 is also known as angiopoietin-like 3, angiopoietin-5 (ANGPT5), ANG-5, and FHBL2.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human ANGPTL3 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human ANGPTL3 protein in serum, EDTA plasma, and cell culture supernatants.

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Features

  • Capable of measuring human ANGPTL3 protein
  • Sensitivity: 0.08 ng/mL

Applications

  • Measuring human ANGPTL3 protein in serum, EDTA plasma, and cell culture supernatants

Components

Precoated plate: Anti-Human angiopoietin-like3 (45B1) Mouse IgG
MoAb Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human angiopoietin-
like3 (F48A1) Mouse IgG MoAb Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Human Angiopoietin-like 30.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 0.47–30 ng/mL
  • Assay sensitivity: 0.08 ng/mL
  • Assay duration: Approximately 3–3.5 hours
  • Test specimen type: Serum, EDTA plasma, and cell culture supernatants
  • Specificity: Shows ≤0.1% cross-reactivity with human ANGPTL2, ANGPTL4, and ANGPTL6
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Contents Size Price Units Select
27750A Human ANGPTL3 (highly sensitive) Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

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