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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_A-D >  ANGPTL2_Human

Human ANGPTL2 ELISA Kit

Angiopoietin-like protein 2 (ANGPTL2) is a secreted glycoprotein that has strong structural similarity to angiopoietins. It contains a signal peptide, N-terminal coiled-coil domain, and a C-terminal fibrinogen (FBN)-like domain. ANGPTL2 is believed to induce sprouting in endothelial cells through autocrine and paracrine actions. It has been implicated in the metastasis of tumor cells in mouse models. ANGPTL2 is also known as angiopoietin-like 2, angiopoietin-related protein 2 (ARP2), and HARP.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human ANGPTL2 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human ANGPTL2 protein in serum, EDTA plasma, and cell culture supernatants.

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Features

  • Capable of measuring human ANGPTL2 protein
  • Sensitivity: 0.01 ng/mL

Applications

  • Measuring human ANGPTL2 protein in serum, EDTA plasma, and cell culture supernatants

Components

Precoated plate: Anti-Human ANGPTL2 (K2-1A1A) Mouse
IgG MoAb Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human
ANGPTL2 (K1-12A4A) Mouse IgG MoAb Fab Affinity-Purified
0.4 mL x 1
Standard: Human ANGPTL20.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 0.05–3.5 ng/mL
  • Assay sensitivity: 0.01 ng/mL
  • Assay duration: Approximately 3–3.5 hours
  • Test specimen type: Serum, EDTA plasma, and cell culture supernatants
  • Specificity: Shows ≤0.1% cross-reactivity with human ANGPTL3, ANGPTL4, and ANGPTL6
  • Sample volume per well: 100 µL

 
Products
Offers Cat. # Product Contents Size Price Units Select
27745A Human ANGPTL2 Assay Kit - IBL 96-Well $849.00 *
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