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Products >  Cell_Biology >  Metabolic_Diseases >  Antibodies >  TNF-alpha_Rat

Anti-Rat TNF-alpha Rabbit IgG Antibody

Tumor necrosis factor (TNF, formerly known as TNF-alpha) is a proinflammatory cytokine secreted primarily by macrophages. TNF-alpha belongs to a group of cytokines that regulates the acute-phase response to injury. It is a membrane-integrated protein that normally exists as homotrimers in humans. TNF-alpha binds two receptors, TNF-R1 and TNF-R2. TNF-alpha activates three major signaling pathways, mediated by NF-kappaB, MAP kinases, and the death-signaling cascade. TNF-alpha is a pyrogen that causes fever by direct action or by stimulation of interleukin-1 (IL-1) secretion. It has been implicated in autoimmune diseases, insulin resistance, and cancer. TNF-alpha is also known as TNFA, tumor necrosis factor ligand superfamily member 2 (TNFSF2), DIF, APC1 protein, and cachectin.

This product is an affinity-purified IgG antibody that recognizes rat TNF-alpha protein. The antibody was raised in rabbit using a synthetic peptide and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of rat TNF-alpha protein.

  At-A-Glance   Documents

Features

  • Cat.#: 18285A, 18285B
  • Target Name: Rat TNF-alpha protein
  • Antigen: Rat TNF-alpha C-terminal synthetic peptide
  • Application: WB, IHC
  • Target Entrez Gene ID: 24835

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 5 µg/mL
  • Immunohistochemical techniques: 5 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O (final concentration 100 µg/mL).

Alternative names

TNF, TNFA, tumor necrosis factor ligand superfamily member 2, TNFSF2, DIF, APC1 protein, cachectin




 
 
Products
Offers Cat. # Product Contents Size Price Units Select
18285A Anti-Rat TNF-alpha Rabbit IgG Affinity Purify 10 ug $113.00 *
  18285B Anti-Rat TNF-alpha Rabbit IgG Affinity Purify 100 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 
 

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