Home Takara Clontech Logo Takara Clontech
login | register | order now | view cart | feedback
Products
Cell Biology


Products >  Cell_Biology >  Metabolic_Diseases >  Antibodies >  Endothelin-3

Anti-Endothelin-3 Rabbit IgG Antibody

Endothelins are among the most potent vasoconstrictor proteins known. Three isoforms are known, each containing 21 amino acids: endothelin-1 (ET-1), endothelin-2 (ET-2), and endothelin-3 (ET-3). All three isoforms are encoded by a 38-amino-acid precursor known as big endothelin. Endothelins are expressed in many tissues, including lung, kidney, brain, pituitary, and placenta. The active form of endothelin-3 is a ligand for endothelin receptor type B (EDNRB). This interaction is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in the endothelin-3 gene are associated with the congenital disorders Hirschsprung disease (HSCR) and Waardenburg syndrome (WS). Endothelin-3 is also known as PPET3, HSCR4, and WS4B.

This product is an affinity-purified IgG antibody that recognizes human, mouse, and rat ET-3. The antibody was raised in rabbit using a synthetic peptide, and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of ET-3.

  At-A-Glance   Documents

Features

  • Cat.#: 18202A, 18203B
  • Target Name: Human ET-3
  • Antigen: ET-3 synthetic peptide
  • Application: WB, IHC
  • Specificity: Reacts with human, mouse, and rat ET-3 and big ET-3; shows 0.8% cross-reactivity with ET-1 and ET-2
  • Target Entrez Gene ID: 1908

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 2–5 µg/mL
  • Immunohistochemical techniques: 3–5 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O (final concentration 50 µg/mL)

Alternative names

PPET3, HSCR4, WS4B




 
 
Products
Offers Cat. # Product Contents Size Price Units Select
18202A Anti-Endothelin-3 Rabbit IgG Affinity Purify 5 ug $113.00 *
  18202B Anti-Endothelin-3 Rabbit IgG Affinity Purify 50 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 
 

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions