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Products >  Cell_Biology >  Metabolic_Diseases >  Antibodies >  Big_Endothelin-1

Big Endothelin-1 Detection

Endothelins are among the most potent vasoconstrictor proteins known. Overexpression of endothelins contributes to hypertension and heart disease. Three isoforms are known, each containing 21 amino acids: endothelin-1 (ET-1), endothelin-2 (ET-2), and endothelin-3 (ET-3). All three isoforms are encoded by a 38-amino-acid precursor known as big endothelin. Endothelins are expressed in many tissues, including lung, kidney, brain, pituitary, and placenta.

ELISAs for Big Endothelin-1 Detection

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to human or rat big ET-1 protein; one is precoated on the ELISA plate, and the other is HRP-conjugated. These kits can be used to measure soluble human or rat big ET-1 protein in serum, EDTA-plasma, cell culture supernatants, or tissue extracts.

Antibodies for Big Endothelin-1 Detection

These products are affinity-purified IgG antibodies that recognizes human and rat big ET-1. The antibodies were raised in rabbit using synthetic big ET-1 (22-38) peptides, and can be used for immunohistochemical (IHC) detection of human or rat big ET-1 protein.

  At-A-Glance   Documents

Features

Antigen
Cat. #
Application
Species
(Clone) and Source
 
Cross‑Reactivity
 
Big ET‑1 (22-38)27168AELISAHuman
Mouse
Rat

Capture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG

Cross‑reacts 100% with mouse and rat big ET-1 and ≤ 0.1% against ET-1, ET-3
Big ET‑1 (22-38)27167AELISARat
Human
Mouse

Capture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG

Cross‑reacts 100% with human and mouse big ET-1 and ≤ 0.1% against ET-1, ET-3
Big ET‑1 (22-38)18203A, BIHCHuman(n/a)
Rabbit IgG

<0.1% cross-reactivity with big ET-2, -3, and BIC

Does not cross react with ET-1, -2, or -3

Big ET‑1 (22-38)18205AIHCRat(n/a)
Rabbit IgG

Cross-reacts with human big ET-1

≤0.1% cross-reactivity with big ET-2, -3, and VIC

Does not cross react with ET-1, -2, or -3

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.




 
 
Products
Cat. # Product Contents Size Price Units Select
18203A Anti-Human Big Endothelin-1 (22-38) Rabbit IgG Affinity Purify 5 ug $113.00
18203B Anti-Human Big Endothelin-1 (22-38) Rabbit IgG Affinity Purify 50 ug $499.00
18205A Anti-Rat Big Endothelin-1 (22-38) Rabbit IgG Affinity Purify 50 ug $499.00
 

 

 
 

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