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Products >  Cell_Biology >  Epigenetics >  Antibodies >  RET_Finger_Protein

Anti-RET Finger Protein (RFP) Rabbit IgG Antibody

RET finger protein (RFP) belongs to a large family of proteins characterized by the Really Interesting New Gene (RING) zinc-finger motif. It is also known as tripartite motif-containing protein 27 (TRIM27), RING finger protein 76 (RNF76), RFP transforming protein, and zinc finger protein RFP. RFP is encoded by the TRIM27 gene and is known to become oncogenic by fusion with RET tyrosine kinase. Although RFP is reported to be localized to the nuclear matrix, its function is unknown. In healthy mammals, RFP is expressed at high levels in male germ cells. Two isoforms of RFP, 58 kDa and 68 kDa, are detected in mouse testis. RFP has been proposed to repress transcriptional activity of specific proteins such as Retinoblastoma (Rb) protein or bHLH transcription factors. It also acts as a transcriptional repressor in Drosophila, through interaction with Enhancer of Polycomb (EPC) protein.

This product is an affinity-purified IgG antibody that recognizes human and mouse RFP. The antibody was raised in rabbit using a synthetic peptide, and can be used for Western blot (WB) detection, immunohistochemical (IHC) detection, immunoprecipitation (IP), or immunofluorescence detection of RFP.

At-A-Glance Documents Images & Data

Features

  • Cat.#: 18791A, 18791B
  • Target Name: Human RFP
  • Antigen: Human RFP C-terminal synthetic peptide
  • Application: WB, IHC, IP
  • Specificity: Cross-reacts with mouse RFP
  • Target Entrez Gene ID: 5987

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 1-2 µg/mL
  • Immunoprecipitation: 2 µg/mL
  • Immunofluorescence: 0.1–1.0 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O (final concentration 100 µg/mL).

Alternative names

Tripartite motif-containing protein 27, TRIM27, RING finger protein 76, RNF76, RFP transforming protein, zinc finger protein RFP




 
 
Products
Cat. # Product Package Size Price # of Units Select
18791A Anti-RET Finger Protein (RFP) Rabbit IgG Affinity Purify 10 ug $100.00
18791B Anti-RET Finger Protein (RFP) Rabbit IgG Affinity Purify 100 ug $499.00
 

 



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