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Products >  Cell_Biology >  Cytokines >  ELISA_Kits >  TNF-alpha_Rat_ELISA

Rat TNF-alpha ELISA Kit

Tumor necrosis factor (TNF, formerly known as TNF-alpha) is a proinflammatory cytokine that is secreted primarily by activated macrophages. It is a member of a cytokine superfamily that regulates the acute-phase response to injury, and it acts as a pyrogen that causes fever directly or by stimulating interleukin-1 (IL-1) secretion. In humans, TNF-alpha is a membrane-integrated protein that normally exists as homotrimers. TNF-alpha binds two receptors, TNF-R1 and TNF-R2, and activates three major signaling pathways: the NF-kappaB pathway, the MAP kinase pathway, and the death-signaling (apoptosis) cascade. It has been implicated in autoimmune diseases, insulin resistance, and cancer. TNF-alpha is also known as TNFA, tumor necrosis factor ligand superfamily member 2 (TNFSF2), DIF, APC1 protein, and cachectin.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to rat TNF-alpha protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble rat TNF-alpha protein in cell culture supernatants.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring recombinant or native rat TNF-alpha protein
  • Sensitivity: 10.55 pg/mL

Applications

  • Measuring rat TNF-alpha protein in cell culture supernatants

Components

Precoated plate: Anti-Rat TNF-alpha (C) Rabbit IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Rat TNF-alpha Rabbit
IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Rat TNF-alpha0.5 mL x 2
EIA buffer : 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 56.25–3600 pg/mL
  • Assay sensitivity: 10.55 pg/mL
  • Assay duration: Overnight
  • Test specimen type: Cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27194A Rat TNF- alpha Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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