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Products >  Cell_Biology >  Cytokines >  ELISA_Kits >  Rat_GRO_CINC-1_ELISA

Rat GRO/CINC-1 ELISA Kit

Cytokine-induced neutrophil chemoattractant-1 (CINC-1) is a member of the alpha (CXC) subfamily of chemokines. It is also known as rat growth-regulated protein (GRO), melanoma growth-stimulating activity alpha (MGSA-alpha), neutrophil-activating protein 3 (NAP-3), fibroblast secretory protein (FSP), C-X-C motif chemokine 1, and SCYB1. Other members of the subfamily include CINC-2-alpha, CINC-2-beta, and CINC-3. They exhibit significant sequence similarity to human GROs and mouse MIP-2. CINC-1 possesses high sequence similarity to IL-8, and has almost the same level of leukocyte chemotaxis. It also has high similarity to mouse keratinocyte-derived cytokine (KC).

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to rat CINC-1 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble rat CINC-1 protein in serum, EDTA plasma, or cell culture supernatants.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring recombinant and native rat CINC-1
  • Sensitivity: 0.35 pg/mL

Applications

  • Measuring rat CINC-1 protein in serum, EDTA plasma, or cell culture supernatants

Components

Precoated plate: Anti-Rat GRO/CINC-1 Rabbit IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Rat GRO/CINC-1
Rabbit IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Rat GRO/CINC-10.5 mL x 2
EIA buffer : 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 4.69–300 pg/mL
  • Assay sensitivity: 0.35 pg/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Rat CINC-1; exhibits ≤0.1% cross-reactivity with rat CINC-2-alpha, CINC-2-beta, CINC-3, and mouse GRO-beta/MIP-2
  • Species cross-reactivity: Cross-reacts with mouse CINC-1
  • Test specimen type: Serum, EDTA plasma, or cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27162A Rat GRO/CINC-1 Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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