These MPL ELISA or thrombopoietin ELISA kits enable detection of either mouse c-Myeloproliferative Leukemia protein (c-MPL), also known as Thrombopoietin Receptor (TPOR) or Cluster of Differentiation 110 (CD110), or human thrombopoietin (also known as TPO, THPO, or MGDF). c-MPL is a 635 amino acid long transmembrane protein with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. c-MPL is a receptor for thrombopoietin, which is a critical regulator of platelet formation and megakaryocytopoiesis. Binding of the class I hematopoietic cytokine thrombopoietin causes c-MPL to dimerize and activate the JAK/STAT pathway, thereby controlling a cell signaling pathway leading to production of platelets. c-MPL (TPOR) may also play a role in hematopoietic stem cell maintenance in the bone marrow. The Mouse c-MPL/TPOR Assay Kit (Cat.# 27174A) is a c-MPL ELISA kit that allows sensitive, specific measurements of mouse soluble c-MPL in serum or cell culture supernatant samples. The Human TPO Assay Kit* (Cat.# 27175A) is a human thrombopoietin ELISA kit, and is recommended for use with cell culture supernatant samples.
Several human diseases are associated with mutations in either thrombopoietin or c-MPL. Mutations in THPO are known that result in hereditary thrombocythemia 1 (resulting in high blood platelet count), or some forms of leukemia. In mice, deficiency in c-MPL / TPOR results in severe thrombocytopenia, underscoring the role of thrombopoietin receptor in regulating megakaryocytopoiesis and platelet formation.
Each ELISA kit provides materials needed to perform a solid phase sandwich ELISA using two highly specific antibodies, one precoated on the ELISA multi-well plate as a capture antibody and one HRP-conjugated as a detection antibody. Tetra Methyl Benzidine (TMB) is used as a substrate for detection.
*Cat. # 27175A is not available in China, Taiwan, and Korea.