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Products >  Cell_Biology >  Cytokines >  ELISA_Kits >  MPL

MPL ELISA Assay Kit - Mouse c-MPL and Human TPO (THPO)

These MPL ELISA  or thrombopoietin ELISA kits enable detection of either mouse c-Myeloproliferative Leukemia protein (c-MPL), also known as Thrombopoietin Receptor (TPOR) or Cluster of Differentiation 110 (CD110), or human thrombopoietin (also known as TPO, THPO, or MGDF). c-MPL is a 635 amino acid long transmembrane protein with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. c-MPL is a receptor for thrombopoietin, which is a critical regulator of platelet formation and megakaryocytopoiesis. Binding of the class I hematopoietic cytokine thrombopoietin causes c-MPL to dimerize and activate the JAK/STAT pathway, thereby controlling a cell signaling pathway leading to production of platelets. c-MPL (TPOR) may also play a role in hematopoietic stem cell maintenance  in the bone marrow. The Mouse c-MPL/TPOR Assay Kit (Cat.# 27174A) is a c-MPL ELISA kit that allows sensitive, specific measurements of mouse soluble c-MPL in serum or cell culture supernatant samples. The Human TPO Assay Kit* (Cat.# 27175A) is a human thrombopoietin ELISA kit, and is recommended for use with cell culture supernatant samples.

Several human diseases are associated with mutations in either thrombopoietin or c-MPL. Mutations in THPO are known that result in hereditary thrombocythemia 1 (resulting in high blood platelet count), or some forms of leukemia. In mice, deficiency in c-MPL / TPOR results in severe thrombocytopenia, underscoring the role of thrombopoietin receptor in regulating megakaryocytopoiesis and platelet formation.

Each ELISA kit provides materials needed to perform a solid phase sandwich ELISA using two highly specific antibodies, one precoated on the ELISA multi-well plate as a capture antibody and one HRP-conjugated as a detection antibody. Tetra Methyl Benzidine (TMB) is used as a substrate for detection.

*Cat. # 27175A is not available in China, Taiwan, and Korea.

  At-A-Glance   Documents   Images & Data

Features

c-MPL ELISA Kit (Cat.# 27174A)

  • Capable of measuring mouse c-MPL (TPOR) protein
  • Sensitivity: 3.5 pg/mL

Human TPO Assay Kit (Cat.# 27175A)

  • Capable of measuring human thrombopoietin protein
  • Sensitivity: 17.3 pg/mL

Applications

  • Measuring mouse c-MPL protein in serum or cell culture supernatant samples (Cat.# 27174A)
  • Measuring human THPO / TPO protein in  cell culture supernatant samples (Cat.# 27175A)

Components

Mouse c-MPL/TPOR Assay Kit

Precoated plate: Anti-Mouse c-MPL (AMM2) Rat IgG MoAb Affinity Purify96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Mouse c-MPL
  (41A1A) Mouse IgG Fab’ Affinity Purify
0.4 mL x 1
Standard: Recombinant mouse soluble c-MPL/TPOR0.5 mL x 2
EIA buffer30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution:  1N H2SO412 mL x 1
Wash buffer (40X): Phosphate buffer50 mL x 1

Human TPO Assay Kit

                                 
Precoated plate: Anti-Human TPO Rabbit IgG Affinity Purify96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human TPO
      (9B2) Rabbit IgG Fab’ Affinity Purify
0.4 mL x 1
Standard: Recombinant human TPO0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution:  1N H2SO412 mL x 1
Wash buffer (40X): Phosphate buffer50 mL x 1

Storage

2–8°C

Performance

Mouse c-MPL/TPOR Assay Kit (Cat.# 27174A)

  • Range of assay: 21.88 - 1,400 pg/mL
  • Assay sensitivity: 3.5 pg/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Mouse c-MPL/TPOR. Cross-reactivity with albumin, transferrin, or immunoglobulin < 0.1 %.
  • Test specimen type: Serum or cell culture supernatants
  • Sample volume per well: 100 µL

Human TPO Assay Kit (Cat.# 27175A)

  • Range of assay: 37.5 - 2,400 pg/mL
  • Assay sensitivity: 17.3 pg/mL
  • Assay duration: Approximately 3–3.5 hours
  • Test specimen type: Cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Offers Cat. # Product Contents Size Price Units Select
27174A Mouse c-MPL/TPOR Assay Kit - IBL 96-Well $930.00 *
27175A Human TPO Assay Kit - IBL 96-Well $930.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

Recommended Products
Offers Cat. # Product Contents Size Price Units Select
10401A Anti-Mouse c-MPL/TPOR (AMM2) Rat IgG MoAb 10 ug $113.00 *
  10403A Anti-Mouse c-MPL/TPOR (AMM2) Rat IgG MoAb Biotin 5 ug $113.00 *
18505A Anti-Human c-MPL-P (M617) Rabbit IgG Affinity Purify 25 ug $113.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

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