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Products >  Cell_Biology >  Cytokines >  ELISA_Kits >  MCP-1_Rat_ELISA

Rat MCP-1 ELISA Kit

Monocyte chemoattractant protein-1 (MCP-1) is 76 amino-acid cytokine that belongs to the CC chemokine subfamily. It is also known as macrophage/monocyte chemotactic and activating factor (MCAF), chemokine (C-C motif) ligand 2 (CCL2), small inducible cytokine A2, SCYA2, HC11, GDCF-2, SMC-CF, monocyte secretory protein JE, and monocyte chemotactic protein. MCP-1 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. MCP-1 is implicated in several types of chronic inflammatory diseases, such as psoriasis, rheumatoid arthritis, nephritis, and atherosclerosis.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to rat MCP-1 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble rat MCP-1 protein in serum, urine, or cell culture supernatants.

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Features

  • Capable of measuring recombinant and native rat MCP-1

Applications

  • Measuring rat MCP-1 protein in serum, urine, or cell culture supernatants

Components

Precoated plate: Anti-Rat MCP-1 Rabbit IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Rat MCP-1
Mouse IgG Fab Affinity-Purified
0.5 mL x 1
Standard: Recombinant Rat MCP-10.5 mL x 1
EIA buffer : 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS10.5 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO411 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 50–3200 pg/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Rat MCP-1
  • Species cross-reactivity: Cross-reacts with human and mouse MCP-1
  • Test specimen type: Serum, urine, or cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Offers Cat. # Product Contents Size Price Units Select
17176A Rat MCP-1 Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

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