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Products >  Cell_Biology >  Cytokines >  ELISA_Kits >  IL-6_Rat

Rat IL-6 (Native Form) ELISA Kit

Interleukins are a group of cytokines that were originally identified in white blood cells (leukocytes). It is now known that interleukins are expressed in a wide variety of cells, and are involved in multiple cell signaling pathways. Interleukin 6 (IL-6) is involved in a wide variety of biological functions, including the inflammatory response and the maturation of B cells. IL-6 acts on B cells, T cells, hepatocytes, hematopoietic progenitor cells, and cells of the central nervous system (CNS). IL-6 is expressed at sites of acute and chronic inflammation, where it is secreted into the serum and binds the IL-6 receptor alpha. In humans, the IL-6 gene has been implicated in many inflammation-associated disease states, including diabetes mellitus and juvenile rheumatoid arthritis. IL-6 is also known as interferon beta-2 (IFNB2), B-cell stimulatory factor-2 (BSF-2), CTL differentiation factor (CDF), B-cell stimulatory factor 2 (BSF2), B-cell differentiation factor, and hybridoma growth factor (HGF).

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to the native form of rat IL-6; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble native rat IL-6 protein in EDTA plasma or cell culture supernatants.

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Features

  • Capable of measuring native rat IL-6
  • Sensitivity: 1.38 AU/mL
    Note: The ED50 concentration in a cell growth test on a rat IL-6 dependent cell line was defined as 1 AU/mL. Direct comparison with recombinant rat IL-6 is not recommended, because reactivity with the recombinant and native forms of rat IL-6 differ.

Applications

  • Measuring native rat IL-6 protein in EDTA plasma or cell culture supernatants

Components

Precoated plate: Anti-Rat IL-6 Rabbit IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Rat IL-6 Rabbit
IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Rat IL-60.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 11.72–750 AU/mL
  • Assay sensitivity: 1.38 AU/mL
  • Assay duration: Overnight
  • Specificity: Does not cross-react with rat CINC-1, CINC-2-alpha, CINC-2-beta, CINC-3, MCP-1, IL-1 beta, or TNF alpha
  • Test specimen type: EDTA plasma or cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Offers Cat. # Product Contents Size Price Units Select
27197A Rat IL-6 (Native Form) Assay Kit - IBL 96-Well $606.00 *
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