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Products >  Cell_Biology >  Cytokines >  ELISA_Kits >  IL-6_Mouse

Mouse IL-6 ELISA Kit

Interleukins are cytokines that were originally identified in leukocytes. They are now known to be expressed in a wide variety of cells, and are involved in a variety of cell signaling pathways. Interleukin 6 (IL-6) is a cytokine that mediates a range of biological functions, including the inflammatory response and the maturation of B cells. IL-6 acts on B cells, T cells, hepatocytes, hematopoietic progenitor cells, and cells of the central nervous system (CNS). It is expressed at sites of acute and chronic inflammation, where it is secreted into the serum and binds the IL-6 receptor alpha. The IL-6 gene, in humans, is linked to many inflammation-associated disease states, including diabetes mellitus and juvenile rheumatoid arthritis. IL-6 is also known as interferon beta-2 (IFNB2), B-cell stimulatory factor-2 (BSF-2), CTL differentiation factor (CDF), B-cell stimulatory factor 2 (BSF2), B-cell differentiation factor, and hybridoma growth factor (HGF).

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to mouse IL-6; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble mouse IL-6 protein in serum, EDTA plasma, or cell culture supernatants.

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Features

  • Capable of measuring mouse IL-6
  • Sensitivity: 1.07 pg/mL

Applications

  • Measuring mouse IL-6 protein in serum, EDTA plasma, or cell culture supernatants

Components

Precoated plate: Anti-Mouse IL-6 Rabbit IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Mouse IL-6 Rabbit
IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Mouse IL-60.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 10.94–700 pg/mL
  • Assay sensitivity: 1.07 pg/mL
  • Assay duration: Overnight
  • Specificity: Exhibits ≤0.1% cross-reactivity with mouse leptin, MIP-2, and osteopontin
  • Test specimen type: Serum, EDTA plasma, or cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Contents Size Price Units Select
27768A Mouse IL-6 Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

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