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Products >  Cell_Biology >  Cytokines >  ELISA_Kits >  IL-1_Beta_Rat

Rat IL-1 Beta ELISA Kit

Interleukins are cytokines that were originally identified in leukocytes. They are now known to be expressed in a wide variety of cells, and are involved in a variety of cell signaling pathways. Interleukin 1 (IL-1) alpha and beta participate in the regulation of immune responses, inflammatory reactions, and hematopoiesis. IL-1 beta also plays a role in memory functions and regulation of the central nervous system (CNS), as well as cell proliferation, differentiation, and apoptosis. IL-1 beta is produced primarily in activated macrophages as an inactive precursor that is cleaved by caspase 1 to yield the active cytokine. It acts through two main receptors that also bind IL-1 alpha. IL-1 beta is also known as catabolin, IL1B, and IL1F2.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to rat IL-1 beta protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble rat IL-1 beta protein in serum, EDTA plasma, or cell culture supernatants.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring rat IL-1 beta
  • Sensitivity: 1.67 pg/mL

Applications

  • Measuring rat IL-1 beta protein in serum, EDTA plasma, or cell culture supernatants

Components

Precoated plate: Anti-Rat IL-1 Beta Rabbit IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Rat IL-1 Beta
Rabbit IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Rat IL-1 Beta0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 11.72–750 pg/mL
  • Assay sensitivity: 1.67 pg/mL
  • Assay duration: Overnight
  • Specificity: Exhibits ≤0.1% cross-reactivity with rat IL-1 alpha
  • Test specimen type: Serum, EDTA plasma, or cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27193A Rat IL-1 beta Assay Kit - IBL 96-Well $606.00 *
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