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Products >  Cell_Biology >  Cytokines >  ELISA_Kits >  GRO_KC_Mouse

Mouse GRO/KC ELISA Kit

Mouse keratinocyte-derived cytokine (KC) is the homolog of human chemokine (C-X-C motif) ligand 1 (CXCL1) protein, a small cytokine belonging to the CXC chemokine subfamily. It was previously known as GRO1 oncogene, GRO-alpha, and melanoma growth stimulating activity (MSGA); it is also called growth-regulated alpha protein, secretory protein N51, and platelet-derived growth factor-inducible protein KC. Mouse KC is also homologous to rat CINC-1. KC was originally identified after induction by platelet-derived growth factor (PDGF). Expression of KC is also induced by mitogens in vascular smooth cells, endothelial cells, and macrophages. In mouse, KC activity is mediated by the interleukin-8 (IL-8) receptor, which also binds mouse MIP-2. These two chemokines have been suggested to be mouse homologs of human IL-8.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to mouse KC protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble mouse KC protein in serum, EDTA plasma, or cell culture supernatants.

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Features

  • Capable of measuring recombinant and native mouse KC
  • Sensitivity: 1.27 pg/mL

Applications

  • Measuring mouse KC protein in serum, EDTA plasma, or cell culture supernatants

Components

Precoated plate: Anti-Mouse GRO/KC Rabbit IgG
Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Mouse
GRO/KC Rabbit IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Mouse GRO/KC0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 23.44–1500 pg/mL
  • Assay sensitivity: 1.27 pg/mL
  • Assay duration: Approximately 3-3.5 hours
  • Specificity: Exhibits 0.5% cross-reactivity with rat CINC-2-beta; ≤0.1% with mouse MIP-2, rat CINC-2-alpha, and rat CINC-3
  • Test specimen type: Serum, EDTA plasma, or cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Offers Cat. # Product Contents Size Price Units Select
27137A Mouse GRO/KC Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

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