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Products >  Cell_Biology >  Cytokines >  ELISA_Kits >  GRO_CINC-3_Rat

Rat GRO-beta/CINC-3/CXCL2/MIP-2 ELISA Kit

Rat growth-regulated protein beta (GRO-beta) is a member of the CXC family of chemokines. It is also known as chemokine (C-X-C motif) ligand 2 (CXCL2), cytokine-induced neutrophil chemoattractant-3 (CINC-3), macrophage inflammatory protein 2-alpha (MIP2-alpha), melanoma growth stimulatory activity beta (MGSA-beta), and SCYB2. Rat CINC-3 family members, including CINC-1, CINC-2-alpha, CINC-2-beta, and CINC-3, show significant sequence similarity to human GROs and mouse MIP-2 and KC protein. The CINC chemokines are expressed in monocytes and macrophages, endothelial cells, and fibroblasts. CINC-3 interacts with a cell-surface G-protein-coupled receptor known as CXCR2.

This kit is a solid-phase sandwich ELISAs using two antibodies that are highly specific to rat CINC-3 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure soluble rat CINC-3 protein in serum, EDTA-plasma, or cell culture supernatant.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring rat CINC-3
  • Sensitivity: 0.51 pg/mL

Applications

  • Measuring rat CINC-3 protein in serum, EDTA-plasma, or cell culture supernatant

Components

Precoated plate: Anti-Rat GRO/CINC-3 Rabbit IgG Affinity-Purified96 Well x 1
Labeled target: HRP conjugated Anti-Rat GRO/CINC-3 Rabbit
IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Rat GRO/CINC-30.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 4.69–300 pg/mL
  • Assay sensitivity: 0.51 pg/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Rat CINC-3; does not cross-react with CINC-1, CINC-2-alpha, or CINC-2-beta
  • Species cross-reactivity: Does not cross-react with mouse GRO/KC
  • Test specimen type: Serum, EDTA-plasma, or cell culture supernatant
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27163A Rat GRO/CINC-3 (GRO beta/MIP-2) Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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