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Products >  Cell_Biology >  Cytokines >  ELISA_Kits >  GRO_CINC-2_Rat

Rat GRO/CINC-2 ELISA Kit

Rat cytokine-induced neutrophil chemoattractant-2 (CINC-2) protein is a member of the CXC family of chemokines. The rat CINC family members are homologous to human GRO/CXCL and mouse MIP-2/KC genes. Rat CINC-2-alpha and CINC-2-beta were originally identified as novel neutrophil chemoattractants after carrageenan-induced inflammation in granulation tissue. Mature CINC-2-beta is a 68 amino-acid protein that is generated by cleavage of a 32-residue signal peptide. CINC-2-alpha and CINC-2-beta differ by only three C-terminal residues. CINC-2-alpha and CINC-2-beta have similar chemotactic activity, and they contribute to neutrophil infiltration into inflammatory sites in rats. Other cell types that express CINC-2 proteins include activated macrophages and fibroblasts. Rat CINC-2 is also known as chemokine (C-X-C motif) ligand 3 (CXCL3) and GM160.

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to rat CINC-2 proteins; one is precoated on the ELISA plate and the other is HRP-conjugated. These assay kits can be used to measure soluble rat CINC-2-alpha (Cat.# 17170) or CINC-2-beta (Cat.# 17166) protein in serum or cell culture supernatants.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring recombinant or native rat CINC-2-alpha or CINC-2-beta

Applications

  • Measuring rat CINC-2-alpha or CINC-2-beta proteins in serum or cell culture supernatants

Components

Precoated plate: Anti-Rat GRO/CINC-2 Rabbit IgG
Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Rat
GRO/CINC-2 Rabbit IgG Fab Affinity-Purified
10.5 mL x 1
Standard: Recombinant Rat GRO/CINC-20.5 mL x 1
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS10.5 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO411 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 9.38–600 pg/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Exhibits ≤0.1% cross-reactivity with other rat CINC isoforms, rat MCP-1, rat Rantes, rat MIP-1-alpha, and rat IL-1-beta
  • Test specimen type: Serum or cell culture supernatants
  • Sample volume per well: 100 µL



 
 
Products
Cat. # Product Package Size Price # of Units Select
17166A Rat GRO/CINC-2 beta Assay Kit - IBL 96-Well $575.00
17170A Rat GRO/CINC-2 alpha Assay Kit - IBL 96-Well $575.00
 

 



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