Rat cytokine-induced neutrophil chemoattractant-2 (CINC-2) protein is a member of the CXC family of chemokines. The rat CINC family members are homologous to human GRO/CXCL and mouse MIP-2/KC genes. Rat CINC-2-alpha and CINC-2-beta were originally identified as novel neutrophil chemoattractants after carrageenan-induced inflammation in granulation tissue. Mature CINC-2-beta is a 68 amino-acid protein that is generated by cleavage of a 32-residue signal peptide. CINC-2-alpha and CINC-2-beta differ by only three C-terminal residues. CINC-2-alpha and CINC-2-beta have similar chemotactic activity, and they contribute to neutrophil infiltration into inflammatory sites in rats. Other cell types that express CINC-2 proteins include activated macrophages and fibroblasts. Rat CINC-2 is also known as chemokine (C-X-C motif) ligand 3 (CXCL3) and GM160.
These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to rat CINC-2 proteins; one is precoated on the ELISA plate and the other is HRP-conjugated. These assay kits can be used to measure soluble rat CINC-2-alpha (Cat.# 17170) or CINC-2-beta (Cat.# 17166) protein in serum or cell culture supernatants.