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Products >  Cell_Biology >  Cytokines >  ELISA_Kits >  GRO_Beta_MIP-2_Mouse

Mouse GRO-beta/MIP-2 ELISA Kit

Mouse macrophage inflammatory protein 2 (MIP-2, also known as MIP-2-alpha) is the homolog of human chemokine (C-X-C motif) ligand 2 (CXCL2) protein, a small cytokine belonging to the CXC chemokine subfamily. It was previously known as GRO2 oncogene, GRO-beta, and melanoma growth stimulating activity beta (MSGA-beta). MIP-2 is also homologous to rat CINC-2. MIP-2 is expressed by activated monocytes and neutrophils at sites of inflammation. It has also been shown to control mucosal lymphocyte migration in mice.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to mouse MIP-2 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble mouse MIP-2 protein in serum, EDTA plasma, or cell culture supernatants.

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Features

  • Capable of measuring mouse MIP-2
  • Sensitivity: 0.36 pg/mL

Applications

  • Measuring mouse MIP-2 protein in serum, EDTA plasma, or cell culture supernatants

Components

Precoated plate: Anti-Mouse GRO-beta/MIP-2 Rabbit
IgG Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Mouse
GRO-beta/MIP-2 Rabbit IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Mouse GRO-beta/MIP-20.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 1–64 pg/mL
  • Assay sensitivity: 0.36 pg/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Exhibits ≤0.1% cross-reactivity with rat CINC-2-alpha, CINC-2-beta, and mouse KC
  • Test specimen type: Serum, EDTA plasma, or cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Contents Size Price Units Select
27138A Mouse GRO beta/MIP-2 Assay Kit - IBL 96-Well $606.00 *
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