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Products >  Cell_Biology >  Cytokines >  ELISA_Kits >  GRO_Beta_Human

Human GRO-beta ELISA Kit

Chemokine (C-X-C motif) ligand 2 (CXCL2) is a small cytokine belonging to the CXC chemokine subfamily. It was previously known as GRO2 oncogene, GRO-beta, macrophage inflammatory protein 2-alpha (MIP2-alpha), and melanoma growth stimulating activity beta (MSGA-beta). CXCL2 exhibits 90% identity in amino acid sequence to CXCL1. CXCL2 is expressed by activated monocytes and neutrophils at sites of inflammation. It exhibits chemotactic activity for polymorphonuclear leukocytes and hematopoietic stem cells (HSCs). CXCL2 is known to interact with a cell-surface chemokine receptor, CXCR2. CXCL2 is also known as growth-regulated beta protein, C-X-C motif chemokine 2, SCYB2, MIP2, MIP2A, CINC2A, and GROB.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human CXCL2 (GRO-beta) protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human CXCL2 protein in serum, EDTA plasma, or cell culture supernatants.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring recombinant and native human CXCL2
  • Sensitivity: 2.93 pg/mL

Applications

  • Measuring human CXCL2 protein in serum, EDTA plasma, or cell culture supernatants

Components

Precoated plate: Anti-Human GRO-beta (C) Rabbit IgG
Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Human
GRO-beta Rabbit IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Human GRO-beta0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 19.53–2,500 pg/mL
  • Assay sensitivity: 2.93 pg/mL
  • Assay duration: Overnight
  • Specificity: Exhibits 1.7% cross-reactivity with human GRO-alpha (CXCL1)
  • Test specimen type: Serum, EDTA plasma, or cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27142A Human GRO beta Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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