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Products >  Cell_Biology >  Cytokines >  ELISA_Kits >  GRO_Alpha_MGSA_Human

Human GRO-alpha/MGSA ELISA Kit

Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine subfamily that is expressed by macrophages, neutrophils, and epithelial cells. It was previously known as GRO1 oncogene, GRO-alpha, fibroblast secretory protein (FSP), and melanoma growth stimulating activity alpha (MSGA-alpha). CXCL1 is also known as neutrophil-activating protein 3 (NAP-3), growth-regulated alpha protein, C-X-C motif chemokine 1, SCYB1, and GROA. The rat homolog of human CXCL1 is known as CINC-1. CXCL1 is secreted by human melanoma cells, has mitogenic properties, and is implicated in melanoma pathogenesis. It inhibits the migration of oligodendrocyte precursors during spinal cord development. CXCL1 also acts as a chemoattractant for neutrophils, and is involved in angiogenesis, inflammation, wound healing, and tumorigenesis.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human CXCL1 (GRO-alpha) protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human CXCL1 (GRO-alpha) protein in serum, EDTA plasma, or cell culture supernatants.

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Features

  • Capable of measuring recombinant and native human CXCL1 (GRO-alpha)
  • Sensitivity: 1.07 pg/mL

Applications

  • Measuring human CXCL1 (GRO-alpha) protein in serum, EDTA plasma, or cell culture supernatants

Components

Precoated plate: Anti-Human GRO-alpha/MGSA Rabbit
IgG Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Human GRO-alpha/
MGSA Rabbit IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Human GRO-alpha/MGSA0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 3.13–200 pg/mL
  • Assay sensitivity: 1.07 pg/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Exhibits ≤0.1% cross-reactivity with human IL-8, IP-10, and rat GRO-alpha (CINC-1)
  • Test specimen type: Serum, EDTA plasma, or cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Contents Size Price Units Select
27139A Human GRO alpha/MGSA Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

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