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Products >  Cell_Biology >  Cytokines >  ELISA_Kits >  G-CSF_Human

Human G-CSF ELISA Kit

Granulocyte colony-stimulating factor (G-CSF or GCSF) is a glycoprotein, growth factor, and cytokine produced by a number of different tissues. It is also known as CSF-3, filgrastim, lenograstim, and pluripoietin. G-CSF stimulates the production of granulocytes and stem cells in bone marrow, and their subsequent release into the circulatory system. G-CSF also regulates the differentiation and propagation of neutrophil precursors using a variety of pathways mediated by kinases such as JAK, STAT, MAPK, PI3K and protein kinase B.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human G-CSF protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human G-CSF protein in cell culture supernatants.

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Features

  • Capable of measuring human G-CSF protein
  • Sensitivity: 1.23 pg/mL

Applications

  • Measuring human G-CSF protein in cell culture supernatants

Components:

Precoated plate: Anti-Human G-CSF Mouse IgG MoAb
Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human G-CSF
Rabbit IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Human G-CSF1.0 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 7.81–500 pg/mL (1 hr. at 37°C); 1.95–250 pg/mL (overnight at 4°C)
  • Assay sensitivity: 1.23 pg/mL
  • Assay duration: Approximately 3–3.5 hours, or overnight
  • Specificity: Human G-CSF
  • Test specimen type: Cell culture supernatants, serum, EDTA-plasma
  • Sample volume per well: 100 µL

 
Products
Offers Cat. # Product Contents Size Price Units Select
27131A Human G-CSF Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

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