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Products >  Cell_Biology >  Cytokines >  ELISA_Kits >  CCL8_MCP2_Mouse

Mouse CCL8/MCP-2 ELISA Kit

Chemokine (C-C motif) ligand 8--also known as monocyte chemoattractant protein 2 (MCP-2), HC14, SCYA8, or SCYA10--is a protein encoded by the CCL8 gene. The precursor protein (109 amino acids) is cleaved to produce mature CCL8 (75 amino acids). CCL8 activates many different immune cells, including mast cells, eosinophils, and basophils (implicated in allergic responses), and monocytes, T cells, and NK cells (involved in the inflammatory response). CCL8 acts through binding to several different cell surface chemokine receptors, including CCR1, CCR2B, and CCR5 (one of the major co-receptors for HIV-1).

This kit is a solid-phase sandwich ELISA using two antibodies that are specific to mouse CCL8; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure mouse CCL8 in EDTA-plasma, heparin plasma or serum.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring mouse CCL8
  • Sensitivity of 49.9 pg/mL

Applications

  • Measuring mouse CCL8 in EDTA-plasma, heparin plasma, or serum

Components

Precoated plate: Anti-Mouse CCL8/MCP-2 (81) Rabbit IgG
Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Mouse
CCL8/MCP-2 (33) Rabbit IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Mouse CCL8/MCP-20.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 78.13–5,000 pg/mL
  • Assay sensitivity: 49.9 pg/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Does not cross-react with mouse CCL2/MCP-1 or CCL7/MCP-3
  • Species cross-reactivity: n/a
  • Test specimen type: EDTA-plasma, heparin plasma, or serum
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27773A Mouse CCL8/MCP-2 Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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