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Products >  Cell_Biology >  Cytokines >  Antibodies_H-T >  KC_Mouse

KC (CXCL1) Rabbit IgG Antibody

KC (CXCL1) Rabbit IgG Antibody is an affinity-purified IgG antibody that recognizes mouse keratinocyte chemoattractant (KC) or C-X-C motif chemokine 1 (CXCL1) protein. The anti-mouse KC antibody is raised in rabbit using recombinant KC protein or synthetic peptides and is applicable in Western blot (WB) or immunohistochemical (IHC) detection of mouse KC protein.

Mouse keratinocyte chemoattractant (KC) or C-X-C motif chemokine 1 (CXCL1) was originally identified by overexpression in murine keratinocytes, monocytes, and macrophages following stimulation by platelet-derived growth factor (PDGF) and macrophage colony-stimulating factor (M-CSF). The murine KC gene is homologous to human chemokine C-X-C motif ligand 1 (CXCL1), previously called growth-regulated alpha protein or GRO-alpha or rat CINC-1. Mouse KC is also called CXCL1, C-X-C motif chemokine 1, SCYB1, Fsp and melanoma growth stimulating activity (MSGA). A truncated form of KC, designated KC-T, exhibits dramatically increased biological activities has been described under the name HSF (hematopoietic synergistic factor). KC is involved in chemotaxis and cell activation of neutrophils and monocytes/macrophages. The synthesis of KC in vascular endothelial cells is induced by thrombin. The murine KC receptor is homologous to the interleukin 8 (IL-8) type B receptor. The KC receptor is capable of binding both KC and macrophage inflammatory protein-2 (MIP-2) with high affinity. Studies have shown that KC expression decreases the severity of multiple sclerosis, suggesting that KC may have a neuroprotective function.

At-A-Glance Documents

Features

Cat.#: 18361A, 18361B
  • Target Name: Mouse KC protein
  • Antigen: Mouse KC recombinant protein
  • Specificity: Reacts with mouse KC and rat CINC-1; shows <0.1% cross-reactivity with human IL-8 and CXCL1; does not cross-react with rat CINC-2-alpha, CINC-2-beta, CINC-3, or mouse MIP-2
  • Application: WB, IHC
  • Target Entrez Gene ID: 14825

Cat.#: 18366A

  • Target Name: Mouse KC protein
  • Antigen: Mouse KC C-terminal synthetic peptide
  • Specificity: Reacts with mouse KC and rat CINC-1; shows <0.1% cross-reactivity with human IL-8 and CXCL1; does not cross-react with rat CINC-2-alpha, CINC-2-beta, CINC-3, or mouse MIP-2
  • Application: WB, IHC
  • Target Entrez Gene ID: 14825

Cat.#: 18367A, 18367B

  • Target Name: Mouse KC protein
  • Antigen: Mouse KC N-terminal synthetic peptide
  • Specificity: Reacts with mouse KC and rat CINC-1; shows <0.1% cross-reactivity with human IL-8 and CXCL1; does not cross-react with rat CINC-2-alpha, CINC-2-beta, CINC-3, or mouse MIP-2
  • Application: WB, IHC
  • Target Entrez Gene ID: 14825

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 10–20 µg/mL (Cat.# 18361); 1–5 µg/mL (Cat.# 18366, 18367)
  • Immunohistochemical techniques: 10–20 µg/mL (Cat.# 18361); 5 µg/mL (Cat.# 18366, 18367)

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O.

Alternative names

CXCL1, C-X-C motif chemokine 1, SCYB1, Fsp, melanoma growth stimulating activity, MSGA




 
 
Products
Cat. # Product Package Size Price # of Units Select
18367A Anti-Mouse KC (N) Rabbit IgG Affinity Purify 10 ug $113.00 *
18367B Anti-Mouse KC (N) Rabbit IgG Affinity Purify 100 ug $499.00
18361A Anti-Mouse KC Rabbit IgG 25 ug $113.00 *
18361B Anti-Mouse KC Rabbit IgG 500 ug $499.00
18366A Anti-Mouse KC (C) Rabbit IgG Affinity Purify 100 ug $499.00
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