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Products >  Cell_Biology >  Cytokines >  Antibodies_H-T >  IRF_3_Human

Anti-Human IRF-3 Rabbit IgG Antibody

The interferon regulatory factor (IRF) family of transcription factors control the expression of several interferon genes, and are involved in the JAK-STAT signaling pathway. Interferon regulatory factor-3 (IRF-3) was discovered based on its homology to IRF-1 and IRF-2. IRF3 is produced as an inactive cytoplasmic form that dimerizes and forms a complex with CREB-binding protein, after phosphorylation of Ser-386. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. IRF-3 plays an important role in the innate immune system's response to viral infection. Double-stranded RNA (dsRNA) and toll-like receptor (TLR) signaling can also induce dimerization and phosphorylation of IRF-3. IRF-3 has been shown to induce significant apoptosis in primary macrophages.

These products are affinity-purified IgG antibodies that recognize human IRF-3 protein. The antibodies were raised in rabbit using synthetic peptides, and can be used for Western blot (WB) detection, immunohistochemical (IHC) detection, or immunoprecipitation (IP) of human IRF-3 protein.

At-A-Glance Documents Images & Data

Features

  • Cat.#: 18781A, 18781B
  • Target Name: Human IRF-3 protein
  • Antigen: Human IRF-3 synthetic peptide
  • Application: WB, IHC, IP
  • Target Entrez Gene ID: 3661

  • Cat.#: 18783A, 18783B
  • Target Name: Human IRF-3 protein (phosphorylated)
  • Antigen: Human IRF-3 (phosphorylated) C-terminal synthetic peptide
  • Application: WB
  • Target Entrez Gene ID: 3661

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 1–5 µg/mL (Cat.# 18781); 2 µg/mL (Cat.# 18783)
  • Immunohistochemical techniques: 1–5 µg/mL
  • Immunoprecipitation: 1–5 µg per reaction

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O (final concentration 100 µg/mL).




 
 
Products
Cat. # Product Package Size Price # of Units Select
18781A Anti-Human IRF-3 Rabbit IgG Affinity Purify 10 ug $113.00 *
18781B Anti-Human IRF-3 Rabbit IgG Affinity Purify 100 ug $499.00
18783A Anti-Human IRF-3 (S386 Phosphorylated) Rabbit IgG Affinity Purify 10 ug $113.00 *
18783B Anti-Human IRF-3 (S386 Phosphorylated) Rabbit IgG Affinity Purify 100 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 



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