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Products >  Cell_Biology >  Cytokines >  Antibodies_H-T >  CD25

Detecting CD25 Cells: Anti-CD25 Monoclonal Antibody

CD25 is a component of the Interleukin 2 receptor (IL-2R). CD25 itself ,also known as IL-2R alpha, is a low-affinity receptor for IL2, but forms a high-affinity IL-2 receptor in association with CD122 and CD132 (beta and gamma subunits, respectively). CD25 is expressed in activated T cells and B cells. CD25 expression is used as a marker to identify regulatory T cells (Tregs or CD4+CD25+ cells), cells that modulate the immune response and prevent autoimmunity. Increased CD25 expression in serum has been observed in patients with adult T cell leukemia and patients with malignant lymphoma.

These products are anti-CD25 monoclonal antibodies. The antibodies were raised in mouse and can be used to detect human CD25 cells by Western blot, immunoprecipitation, and flow cytometry.

At-A-Glance Documents Images & Data

Features

Cat. # 10905A

  • Target Name: CD25
  • Antigen: Purified CD25
  • Clone: H-8
  • Application: WB, IP, FCM
  • Target Entrez Gene ID: 3559

Cat. # 10907A, 10907B

  • Target Name: CD25
  • Antigen: Purified CD25
  • Clone: H-48
  • Application: WB, IP, FCM
  • Target Entrez Gene ID: 3559

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically)

  • Western blot: 10 µg/mL
  • Immunoprecipitation: 10 µg
  • FCM: 1 µg/1x105 cells

Storage

Lyophilized: stable 5 years at 2–8°C.
n solution: stable 2 years at –20°C.

 

Form

Lyophilized product from PBS containing 1 % BSA and 0.05 % NaN3

Alternative Names

IL2RA, CD25, IL2R, TCGFR, IDDM10




 
 
Products
Cat. # Product Package Size Price # of Units Select
10905A Anti-CD25 (H-8) Mouse IgG MoAb 100 ug $595.00
10907A Anti-CD25 (H-48) Mouse IgG MoAb 100 ug $595.00
10907B Anti-CD25 (H-48) Mouse IgG MoAb 500 ug $985.00
 

 



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