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Products >  Cell_Biology >  Cytokines >  Antibodies_A-C >  COX-2_Antibody

Anti-COX-2 Antibody

Cyclooxygenase (COX) is a membrane-bound enzyme that exists as two different isoforms: a constitutively expressed form (COX-1) and an inducible form (COX-2). Also known as prostaglandin endoperoxide H synthase 2 (PHS-2 or PGHS-2), PTGS2, PHS II, GRIPGHS, and Tis10, COX-2 converts arachidonic acid to prostaglandin endoperoxide H2 in a two-step reaction. Levels of COX-2 are elevated during inflammation. COX-2 expression is also upregulated in many forms of cancer. Nonsteroidal anti-inflammatory drugs (NSAIDs) inhibit prostaglandin production by both COX-1 and COX-2.

These products are affinity-purified IgG antibodies that recognize human or rat COX-2. The antibodies were raised in rabbit or mouse using synthetic peptides, and can be used for Western blot (WB) detection, immunohistochemical (IHC) detection, or immunoprecipitation (IP) of human or rat COX-2 protein.

At-A-Glance Documents Images & Data

Features

  • Cat.#: 10211A, 10211B
  • Target Name: Human COX-2
  • Antigen: Human COX-2 synthetic peptide
  • Clone: 13H14
  • Application: WB, IHC, IP
  • Specificity: Does not cross-react with human COX-1
  • Target Entrez Gene ID: 5743

  • Cat.#: 18515A, 18515B
  • Target Name: Human COX-2
  • Antigen: Human COX-2 synthetic peptide
  • Clone: C295
  • Application: WB, IHC
  • Specificity: Does not cross-react with human COX-1 or rat COX-2
  • Target Entrez Gene ID: 5743

  • Cat.#: 18516A, 18516B
  • Target Name: Human COX-2
  • Antigen: Human COX-2 synthetic peptide
  • Clone: C631
  • Application: WB, IHC
  • Specificity: Does not cross-react with human COX-1 or rat COX-2
  • Target Entrez Gene ID: 5743

  • Cat.#: 18525A, 18525B
  • Target Name: Rat COX-2
  • Antigen: Rat COX-2 synthetic peptide
  • Clone: C580
  • Application: WB
  • Specificity: Does not cross-react with human COX-2 or rat COX-1
  • Target Entrez Gene ID: 29527

  • Cat.#: 18955A
  • Target Name: Rat COX-2
  • Antigen: Rat COX-2 synthetic peptide
  • Application: WB, IHC
  • Target Entrez Gene ID: 29527

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 2–5 µg/mL (Cat.# 10211, 18515, 18516, 18525); 5 µg/mL (Cat.# 18955)
  • Immunohistochemical techniques: 2–4 µg/mL (Cat.# 10211); 2–10 µg/mL (Cat.# 18515); 2–5 µg/mL (Cat.# 18516); 5 µg/mL (Cat.# 18955)
  • Immunoprecipitation: 2–5 µg/mL (Cat.# 10211)

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O.

Alternative names

prostaglandin endoperoxide H synthase 2, PHS-2, PGHS-2, PTGS2, PHS II, GRIPGHS, Tis10




 
 
Products
Cat. # Product Package Size Price # of Units Select
10211A Anti-Human COX-2 (13H14) Mouse IgG MoAb 10 ug $100.00
10211B Anti-Human COX-2 (13H14) Mouse IgG MoAb 100 ug $499.00
18515A Anti-Human COX-2 (C295) Rabbit IgG Affinity Purify 10 ug $100.00
18515B Anti-Human COX-2 (C295) Rabbit IgG Affinity Purify 100 ug $499.00
18516A Anti-Human COX-2 (C631) Rabbit IgG Affinity Purify 10 ug $100.00
18516B Anti-Human COX-2 (C631) Rabbit IgG Affinity Purify 100 ug $499.00
18525A Anti-Rat COX-2 (C580) Rabbit IgG Affinity Purify 10 ug $100.00
18525B Anti-Rat COX-2 (C580) Rabbit IgG Affinity Purify 100 ug $499.00
18955A Anti-Rat COX-2 Rabbit IgG Affinity Purify 100 ug $499.00
 

 



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