The Premix WST-1 Cell Proliferation Assay System enables fast and easy colorimetric analysis of cell proliferation and cell viability in a 96-well plate format. This assay depends on the cleavage of the tetrazolium salt WST-1 to form a dark red, soluble formazan dye product via the action of mitochondrial succinate-tetrazolium reductase found in viable cells. As a cell population increases, so does the amount of succinate-tetrazolium reductase, resulting in a concomitant increase in the conversion of WST-1 to formazan dye. Thus, the quantity of formazan dye is directly proportional to the number of metabolically active cells in cell culture medium. Formazan dye formed by metabolically active cells can be quantified by measuring its absorbance height at 440 nm using a microtiter plate reader.
The WST-1 Assay provides a non-radioactive and spectrophotometric method for determining cell proliferation and cell viability without the need to perform any cell harvesting, washing or solubilization. The assay can also be performed in a single multi-well plate. The Premix WST-1 Cell Proliferation Assay System is supplied as a single, ready-to-use solution containing WST-1 and an electron coupling reagent diluted in sterile phosphate buffered saline. Premix WST-1 should be diluted 1:10 in 100-200 µL cultured cells/well. The WST-1 Assay supplies sufficient reagent for 2,500 assays.