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Products >  Cell_Biology >  Cell_Proliferation_and_Viability >  EIA_Kits >  LDH_Cytotoxicity_Detection_Kit

LDH Cytotoxicity Detection Kit

Lactate dehydrogenase (LDH, LD) is a stable cytoplasmic enzyme present in most cells. During damage to (or rupture of) cell cytoplasmic membranes, LDH is released from cells and into the surrounding cell culture supernatant. Quantitation of LDH in cell culture supernatant is one method by which investigators analyze cell death levels.

The LDH Cytotoxicity Detection Kit is a simple yet sensitive, non-radioactive 96-well assay intended for the detection of lactate dehydrogenase (LDH, LD) that is released from damaged cells and into the cell culture supernatant. Via this kit, the LDH that is present in the surrounding cell culture medium participates in a coupled reaction that converts yellow tetrazolium salt (INT) into a red formazan product. The amount of LDH enzyme activity, measured as a 490/492 nm absorbance reading on a microplate reader, directly correlates with the number of damaged cells in culture. The entire assay procedure, which includes cell growth, chemical reaction and absorbance measurement, is performed in a single 96-well plate.

 

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Features

  • Safety: No radioactive isotopes required
  • Accuracy: Assay results strongly correlate with the number of damaged cells
  • High sensitivity: Low cell numbers (e.g., 0.2–2 x 102 cells/well) are easily detected.
  • Fast: Only 0.5–1 hour required for measurement
  • Simple: Relabeling and washing steps not required. Radiation disposal and safety procedures not required. 

Applications

  • Detection of lactate dehydrogenase (LDH, LD) released from damaged cells
  • LDH cytotoxicity detection

Components

Catalyst (diaphorase/ NAD+; lyophilized)5 bottles
Dye solution
(iodotetrazolium chloride (INT) and sodium lactate)
5 x 45 mL

Storage

–20°C

Performance Characteristics

  • Range of assay: 0.2–2 x 102 cells/well
  • Assay sensitivity: 0.2 cells/well
  • Assay duration: 0.5–1 hour
  • Test specimen type: Cell-free culture media (serum-free), cell extract
  • Sample volume per well: 200 µL

    References

    1. Tsuchiya, K. et al. (2005) Infect. Immun. 73(7):3869-3877.

    2. Tsukahara, T. et al. (2004) Cancer Res. 64(15):5442-5448.

    3. Lee, M.J. et al. (2003) J. Immunol. 170(5):2557-2563.

    4. Ito, Y. et al. (2003) Infect. Immun. 71(1):234-241.

    5. Kikuchi, T. et al. (2002) Blood 100(12):3950-3959.

    6. Kohda, C. et al. (2002) Infect. Immun. 70(3):1334-1341.

    7. Baba, H. et al. (2002) Infect. Immun. 70(1):107-113.

    8. Noda, M. et al. (2002) J. Biol. Chem. 277(44):41817-41826.

    9. Zhang, H.M. et al. (2001) Exp. Biol. Med. 226(6):597-604.

    10. Tabrizi, S.J. et al. (2000) Hum. Mol. Genet. 9(18):2683-2689.




     
     
    Products
    Offers Cat. # Product Contents Size Price Units Select
    MK401 LDH Cytotoxicity Detection Kit 2,000 Tests $326.00
     

     

     
     

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