Takara
login | register | order now | view cart  
Products
Cell Biology


Products >  Cell_Biology >  Cell_Proliferation_and_Viability >  EIA_Kits >  Heme_Oxygenase-1_Monoclonal

Heme Oxygenase-1 EIA Kit (Mouse)

Mouse Heme Oxygenase-1 EIA Kit is a sandwich ELISA kit that uses 2 types of antibodies that recognize mouse heme oxygenase-1 and provides a convenient and highly sensitive method for detecting heme oxygenase-1 expressed in response to stress induction.

Heme oxygenase is known to exist as at least two isozymes (heme oxyganase-1 and heme oxganase-2). While heme oxgenase-2 is a consititutive enzyme, heme oxiganase-1(HO-1) is an enzyme whose expression is induced intracellularly in response to various type of stress. Thus, monitoring of heme oxygenase-1 is expected to be effective to detect stress. The Monoclonal Antibodies

This kit uses clone GTS-1 (Cat.# M174) as solid antibody and polyclonal Anti-Heme oxygenase-1 as labeled antibody.

At-A-Glance Documents

Features

  • Sandwich ELISA for the quantitative determination of heme oxygenase-1 expressed in response to stress induction

Applications

  • Quantitative determination of mouse Heme Oxygenase-1 in mouse cell culture supernatants, mouse serum and mouse organ extracts

Components

Antibody Coated Microtiter Plate 1 plate (8 well x 12 strips)
Antibody-Biotin Conjugate (lyophilized)1 vial (for 1 x 11 mL)
Standard 1 vial (for 1 x 1 mL)
Sample Diluent 2 vials (2 x 11 mL)
Avidin-POD Conjugate1 vial (for 1 x 11 mL)
Substrate Solution 1 vial (1 x 12 mL)
Extraction Buffer 1 vial (1 x 11 mL)

Storage

2–8°C

Performance Characteristics

  • Range of assay: 0.125–8.0 ng/mL
  • Assay sensitivity: 0.125 ng/mL
  • Assay duration: 2.5 hours
  • Specificity: This kit specifically measures mouse HO-1 with no detectable cross reaction with mouse HO-2. It cannot be used for human, rabbit or guinea pig. This kit can also be used to measure rat HO-1 with rat HO-1 standard. But the MK124 kit is the most appropriate to measure rat HO-1 in a rat sample.
  • Test specimen type: Mouse serum, organ extracts, cell culture supernatant.
  • Specimen volume required: If each test sample is run in duplicate, approimately 200 µL is required.



 
 
Products
Cat. # Product Package Size Price
MK125 Mouse Heme Oxygenase-1 EIA Kit 96 Assays $567.00
 

 

Recommended Products
Cat. # Product Package Size Price
M174 Monoclonal Anti-Heme Oxygenase-1 (Clone GTS-1) 0.1 mg $350.00
 

 



Clontech is a Takara Bio Company © 2012 Privacy Policy | Terms & Conditions | TRUSTe