Takara
login | register | order now | view cart | feedback
Products
Cell Biology


Products >  Cell_Biology >  Cell_Proliferation_and_Viability >  EIA_Kits >  Heme_Oxygenase-1_EIA_Kit-Rat

Rat Heme Oxygenase-1 EIA Kit (Precoated)

Heme oxygenase-1 (HO-1) is a microsomal enzyme that degrades heme, a prosthetic group of the heme protein family (e.g. hemoglobin), into the bile pigment biliverdin. Biliverdin is subsequently converted to bilirubin, carbon monoxide and reduced iron. Bilirubin has an anti-inflammatory effect and is part of the oxidative stress response in the body due to its strong free radical scavenging activity, whereas carbon monoxide has a vasodilatory effect on organ blood flow.

Heme oxygenase exists in at least two isozyme forms, heme oxygenase-1 and heme oxygenase-2, each of which differs in its tissue expression and inducibility. HO-1 in particular is intracellularly induced as part of the oxidative stress response; therefore, HO-1 monitoring may prove useful for the identification of physiological stress inducers.

The Rat Heme Oxygenase-1 EIA Kit (Precoated) is a 96-well format in vitro enzyme immunoassay kit intended for the quantitation of rat heme oxygenase-1 in rat blood, cell culture supernatant and various organ systems. It is a solid phase sandwich heme oxygenase-1 (HO-1) ELISA that utilizes two mouse monoclonal HO-1 antibodies, one of which is coated on the plate and the other of which is peroxidase-labeled. Detection of rat HO-1 occurs via a two-step incubation method: In the first step, experimental samples are incubated in the antibody-coated microtiter plate. During the second step, the plate is washed and incubated with the peroxidase-labeled HO-1 antibody. The reaction between the peroxidase and the substrate (H2O2, TMBZ) results in color development. The amount of sample HO-1 is determined by measuring signals using an EIA absorbance plate reader. Accurate HO-1 sample concentrations can be determined by comparing their absorbances to the absorbance generated for the supplied standard (via a plotted standard curve).

At-A-Glance Documents Images & Data

Features

  • Heme Oxygenase-1 (HO-1) ELISA
  • Quantitation of HO-1 in rat blood, cell culture supernatant and organs
  • Sensitive detection of as little as 0.125 ng/mL

Applications

  • Quantitation of rat heme oxygenase-1

Components

Antibody-coated microtiter plate 1 plate (8 wells x 12 strips)
Antibody-peroxidase conjugate (lyophilized)1 vial (for 1 x 11 mL)
Standard (lyophilized) 1 vial (for 1 x 1 mL)
Sample diluent 2 vials (2 x 11 mL)
Substrate solution 1 vial (1 x 12 mL)
Extraction buffer1 vial (1 x 11 mL)

Storage

2–8°C

Performance Characteristics

  • Range of assay: 0.125-8.0 ng/mL
  • Assay sensitivity: 0.125 ng/mL
  • Assay duration: 2.5 hours
  • Specificity: Rat HO-1.
  • No cross-reactivity with rat HO-2
  • No cross-reactivity with human, rabbit, guinea pig or mouse HO-1
  • Sample types: Rat serum, organ extract or cell culture supernatant
  • Sample volume per well: 100 µL



 
 
Products
Cat. # Product Package Size Price # of Units Select
MK124 Rat Heme Oxygenase-1 EIA Kit 96 Assays $578.00
 

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
M175 Monoclonal Anti-Heme Oxygenase-1 (Clone GTS-3) 0.1 mg $357.00
M174 Monoclonal Anti-Heme Oxygenase-1 (Clone GTS-1) 0.1 mg $357.00
 

 


Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions