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Products >  Cell_Biology >  Cell_Adhesion_and_Extracellular_Matrix >  EIA_Kits >  Retronectin_EIA_Kit

RetroNectin EIA Kit

RetroNectin is a recombinant human fibronectin (CH-296) that consists of three functional domains: the human fibronectin cell-binding (C-domain), heparin-binding (H-domain) and CS-1 sequence domains. RetroNectin-containing recombinant retroviral vector is often used to improve transduction efficiency during mammalian cell gene transfer. This occurs because the RetroNectin molecule enables close proximity between the H-domain-binding retroviral vector and the transduced animal cell, which has a strong affinity for the RetroNectin C-domain. Additionally, RetroNectin is an effective coating matrix for instruments and labware used to expand ex vivo lymphocytes.

The RetroNectin EIA kit is a sandwich RetroNectin ELISA that takes advantage of plate-coated RetroNectin-specific monoclonal capture antibody to detect and quantitate RetroNectin. The RetroNectin EIA Kit does not cross-react with human fibronectin. Furthermore, RetroNectin monoclonal antibody cross-reactivity is not inhibited even in the presence of human blood components, enabling easy determination of RetroNectin levels. Takara's RetroNectin ELISA also allows highly sensitive quantitation of RetroNectin in cell cryopreservation solutions as well as in cell culture fluids and washes. 

At-A-Glance Documents Images & Data

Features

  • Quantitation of RetroNectin in cryopreservation solutions, cells culture fluids and washes.
  • Sensitive detection of as little as 3.1 ng/mL RetroNectin.

Applications

  • Quantitation of RetroNectin
  • RetroNectin ELISA

Components

Antibody-coated microtiter plate 1 plate (96 wells: 8 wells × 12 strips)
Antibody-peroxidase conjugate (lyophilized)for 11 mL
Standard (lyophilized)for 1 mL
Sample diluent11 mL × 2
Substrate solution (TMBZ)12 mL x 1

Storage

4°C

Performance

  • Range of assay: 3.1–200 ng/mL
  • Sensitivity: 3.1 ng/mL
  • Assay duration: 2.5 hours
  • Specificity: RetroNectin
  • Test specimen type: Cryopreservation solutions, cell culture fluids and washes.
  • Specimen volume required: 100 µL

References

Donahue, R.E. et al. (2001) Mol. Ther. 3:359–367.



 
 
Products
Cat. # Product Package Size Price # of Units Select
MK140 RetroNectin® EIA Kit 96 Assays $653.00
 

 



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