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Products >  Cell_Biology >  Cell_Adhesion_and_Extracellular_Matrix >  EIA_Kits >  Glycocalicin_EIA_Kit

Glycocalicin EIA Kit

Glycocalicin EIA Kit, Ver. 20-52 is a sandwich-based assay for the quantification of human glycocalicin. It is useful for monitoring platelet membrane destruction in human platelet extracts, serum and plasma and to check membrane integrity of stored platelet concentrates over time.

Glycocalicin (GC), a soluble 110 to 135 kDa proteolytic fragment of the platelet membrane glycoprotein Ib (GPIb) alpha-chain, is generated by cleavage of GPIb by Calpain (a Ca2+-dependent protease) present in platelets. Following cleaveage, glycoclicin is released into the plasma. GPIb binds von Willebrand factor and is also a receptor for thrombin. Glycocalicin is considered to be a specific in vivo marker of platelet membrane damage and can be an indicator of platelet activation.

At-A-Glance Documents

Features

  • Sandwich ELISA: for the quantification of human glycocalicin
  • Sensitive: detect as little as 10 ng/mL

Applications

  • Quantitative determination of human glycocalicin in platelet extract, plasma and serum

Components

Antibody Coated Microtiter plate1 plate (8 well × 12 strips)
Antibody-POD Conjugate (PL52-4)1 vial (for 11 mL
Standard1 vial (640 ng)
Sample Diluent2 vials (2 x 11 mL)
Substrate Solution (TMBZ)1 vial (12 mL)

Storage

2–8°C

Properties

  • Range of assay: 10–640 ng/mL
  • Sensitivity: 10 ng/mL
  • Assay duration: 2.5 hours for assay protocol
  • Specificity: This kit specifically measures human Glycocalicin
  • Test specimen type: Human platelet extract, plasma, serum. If each test sample is run in duplicate, about 210 µL (100 µL per assay well plus approx. 10 µL for waste per transfer) is required.

References

  1. Solum, N.O. et al., (1980) Biochem. Biophys. Acta. 597, 235.
  2. Okumura, T. et al., (1976) Thromb. Res. 8, 701.
  3. Coller, B.S. et al., (1983) Blood 61, 99.
  4. Ganguly, P. et al., (1979) Br. J. Haematol. 42, 137.
  5. Kunicki, T.J. et al., (1978) J. Clin. Invest. 62, 716.
  6. Coller, B.S. et al., (1984) J. Clin. Invest. 73, 794.
  7. Steinberg, M.H. et al., (1987) N. Eng. J. Med. 317, 1037.
  8. Kunishima, S. et al., (1991) Clin. Chem. 37, No.2, 169.
  9. Bessos, H. et al., (1990) Thromb. Res. 59, 497.
  10. Bessos, H. et al., (1993) Transfusion Med. 3, 115.



 
 
Products
Cat. # Product Package Size Price
MK105 Glycocalicin EIA Kit, Ver. 20-52 96 Rxns $525.00
 

 


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