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Products >  Cell_Biology >  Cell_Adhesion_and_Extracellular_Matrix >  EIA_Kits >  Galectin-3-binding_protein

Human Galectin-3-binding protein ELISA

Galectin-3-binding protein (also known as LGALS3BP, Mac-2bp, or 90K) is a N-glycosylated, secreted ligand of Galectin-3. Galectin proteins contain carbohydrate-recognition-binding domains (CRDs) that bind beta-galactoside sugars and are involved in mediating cell-cell and cell-matrix interactions. It is thought that LGALS3BP may regulate cell adhesion through interaction with Galectin-3. LGALS3BP has been found to be elevated in the serum of cancer patients and patients infected with HIV.

This kit is a solid phase sandwich ELISA that uses two highly specific antibodies to detect human LGALS3BP (Mac-2bp); one is precoated on the ELISA plate, and the other is HRP-conjugated. This kit can be used to measure human LGALS3BP (Mac-2bp) in serum, EDTA-plasma, or cell culture supernatant samples.

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Features

  • Capable of measuring human Mac-2bp (LGALS3BP, Entrez Gene ID: 3959)
  • Sensitivity: 0.08 ng/mL

Applications

  • Measuring human Mac-2bp (LGALS3BP) in serum, EDTA-plasma, and cell culture supernatants 

Components

Precoated plate: Anti-Human Mac-2bp (8A2) Mouse IgG MoAb Affinity Purify96 Well x 1
Labeled antibody (30X): HRP conjugated Anti- Human Mac-2bp (67A1)
     Mouse IgG MoAb Affinity Purify
0.4 mL x 1
Standard : Recombinant Human Mac-2 binding protei0.5 mL x 2
EIA buffer50 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution:  1N H2SO412 mL x 1
Wash buffer (40X): Phosphate buffer50 mL x 1

Storage

2 - 8°C

Performance

  • Range of assay: 0.78 - 100 ng/mL
  • Assay sensitivity: 0.08 ng/mL
  • Assay duration: approximately 2.5 hours
  • Test specimen type: serum, EDTA-plasma, and cell culture supernatants
  • Sample volume per well: 100 µL

Alternative Names

90K, BTBD17B, MAC-2-BP, TANGO10B


 
Products
Offers Cat. # Product Contents Size Price Units Select
27362A Human Mac-2 Binding Protein (Mac-2bp) Assay Kit - IBL 96-Well $930.00 *
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