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Products >  Cell_Biology >  Cell_Adhesion_and_Extracellular_Matrix >  EIA_Kits >  Fibronectin_EIA_Kit

Fibronectin EIA Kit

Fibronectin (FN) is a high molecular weight (440 kDa) extracellular matrix glycoprotein that is widely distributed throughout the cell surface, plasma and extracellular matrix. FN is involved in many cellular processes, including cell adhesion, growth, migration and differentiation. Published reports indicate that increased levels of fragmented FN or FN-like protein can be observed in the serum, plasma or other biological fluids of cancer patients as compared to control test subjects.

The Fibronectin EIA Kit is a 96-well in vitro enzyme immunoassay kit intended for the quantitative determination of soluble human fibronectin (FN) in serum, urine, cell culture supernatant and other biological fluids. This solid phase fibronectin ELISA utilizes two mouse monoclonal FN antibodies, one of which is coated onto the plate and the other of which is peroxidase-labeled for detection of FN. A two-step incubation method is employed: In the first step, samples are incubated in the antibody-coated microtiter plate; during the second step, the plate is washed and incubated with the peroxidase-labeled FN antibody. A substrate (H2O2, TMBZ) is added, and the reaction between the peroxidase and the substrate results in the development of color. The amount of sample FN is determined by measuring absorbance using an EIA plate reader. Accurate FN sample concentrations can be determined by comparing their specific absorbances with the absorbance obtained from a standard curve (using the supplied kit standard).

At-A-Glance Documents Images & Data Resources

Features

  • Quantitative determination of soluble human FN
  • Detection of as little as 25 ng/mL

Applications

  • Quantitative measurement of serum human fibronectin
  • Fibronectin ELISA

Components

Antibody-coated microtiter plate1 plate (8 wells x 12 strips)
Antibody-peroxidase conjugate (lyophilized)1 vial (for 1 x 11 mL)
Standard (lyophilized)1 vial (for 1 x 11 mL)
Sample diluent2 vials (2 x 11 mL)
Substrate solution1 vial (1 x 12 mL)

Storage

2–8°C

Performance Characteristics

  • Assay range: 25-800 ng/mL
  • Assay sensitivity: 25 ng/mL
  • Assay duration: 2.5 hours
  • Specificity: Human FN.
  • No cross-reactivity with vitronectin, laminin and collagen types I and III
  • No cross-reactivity with mouse, bovine, rabbit and porcine FN
  • Test specimen types: Plasma, serum, urine, culture supernatant and cell extract
  • Sample volume per well: 100 µL

References

1. Liegibel U.M. et al. (2002) J. Exp. Med. 196(10):1387-92.

2. Katayama, M. et al. (1989) Exp. Cell. Res. 185:229-236.

3. Katayama, M. et al. (1991) Clin. Chem. 37:466-471.




 
 
Products
Cat. # Product Package Size Price # of Units Select
MK115 Fibronectin EIA Kit 96 Assays $540.00
 

 

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