Fibronectin (FN) is a high molecular weight (440 kDa) extracellular matrix glycoprotein that is widely distributed throughout the cell surface, plasma and extracellular matrix. FN is involved in many cellular processes, including cell adhesion, growth, migration and differentiation. Published reports indicate that increased levels of fragmented FN or FN-like protein can be observed in the serum, plasma or other biological fluids of cancer patients as compared to control test subjects.
The Fibronectin EIA Kit is a 96-well in vitro enzyme immunoassay kit intended for the quantitative determination of soluble human fibronectin (FN) in serum, urine, cell culture supernatant and other biological fluids. This solid phase fibronectin ELISA utilizes two mouse monoclonal FN antibodies, one of which is coated onto the plate and the other of which is peroxidase-labeled for detection of FN. A two-step incubation method is employed: In the first step, samples are incubated in the antibody-coated microtiter plate; during the second step, the plate is washed and incubated with the peroxidase-labeled FN antibody. A substrate (H2O2, TMBZ) is added, and the reaction between the peroxidase and the substrate results in the development of color. The amount of sample FN is determined by measuring absorbance using an EIA plate reader. Accurate FN sample concentrations can be determined by comparing their specific absorbances with the absorbance obtained from a standard curve (using the supplied kit standard).