Fibronectin EIA Kit

The human Fibronectin EIA Kit is a 96-well in vitro enzyme immunoassay kit for quantitative determination of soluble human fibronectin (FN) in serum, urine, cell culture supernatants, and other biological fluids. It is a solid phase sandwich EIA that utilizes two mouse monoclonal FN antibodies (one of which is coated on the plate, and the other is POD-labeled) for detection of fibronectin using a two-step incubation method. In the first step, samples are incubated in the antibody-coated microtiter plate. During the second step, the plate is washed and incubated with the POD-labeled FN antibody. A substrate is added, and the reaction between POD and the substrate (H₂O₂, TMBZ) results in a color development. The amount of sample FN is determined by measuring absorbance using an EIA plate reader. Accurate FN sample concentrations can be determined by comparing their specific absorbances with the absorbance obtained for the Standard plotted on a standard curve.