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Products >  Cell_Biology >  Cell_Adhesion_and_Extracellular_Matrix >  EIA_Kits >  E-cadherin_EIA_Kit-Human

Human E-cadherin EIA Kit

E-cadherin, also known as uvomorulin, cell-CAM 120/80 or L-CAM, is a calcium-dependent cell adhesion molecule found on epithelial cells in a variety of embryonic and adult tissues. Research studies indicate that unstable or reduced expression of E-cadherin correlates with cancer progression and specifically in cases of lung, breast and hepatocellular carcinoma and gastric and prostate tumors. A soluble 80 kDa form of E-cadherin, presumed to be a degradation product of the intact 120 kDa form, has been reported as elevated in the biological fluids of some cancer patients when compared against healthy individuals. 

The Human E-cadherin EIA Kit is a 96-well in vitro enzyme assay intended for the quantitative determination of soluble human E-cadherin in serum, urine, cell culture supernatant and other biological fluid. It is a solid phase E-cadherin (CDH1) ELISA that utilizes two mouse monoclonal E-cadherin antibodies, one of which is coated onto the plate, and the other of which is peroxidase-labeled. This assay design allows for detection of soluble human E-cadherin via the following two-step incubation method: In the first step, biological samples are incubated in the antibody-coated microtiter plate, while in the second step, the plate is washed and incubated with the peroxidase-labeled E-cadherin antibody. A substrate (H2O2, TMBZ) is added and the reaction between the peroxidase and the substrate results in the development of color. Total soluble E-cadherin is determined by absorbance measurement via an ELISA plate reader. Accurate soluble E-cadherin sample concentration can be determined by comparing its specific absorbance with the absorbance obtained on a standard curve.

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Features

  • Capable of detecting as little as 100 ng/mL
  • Quantitative sandwich ELISA
  • E-cadherin (CDH1) ELISA

Applications

  • Quantitative measurement of soluble human E-cadherin in cultured cells, cell culture supernatants or human biological samples

Components

Antibody-coated microtiter plate1 plate (8 wells x 12 strips)
Antibody-peroxidase conjugate (lyophilized)1 vial (for 1 x 11 mL)
Standard (lyophilized)1 vial (for 1 x 1 mL)
Sample diluent2 vials (2 x 11 mL)
Substrate solution1 vial (1 x 12 mL)

Storage

2–8°C

Performance Characteristics

  • Range of assay: 84.4-2,700 ng/mL
  • Assay sensitivity: 100 ng/mL
  • Assay duration: 3.5 hours
  • Specificity: Human E-cadherin
  • Species cross-reactivity: No reactivity with mouse E-cadherin. Other sources have not been tested.
  • Test specimen type: Serum, urine, culture supernatant and cell extract
  • Sample volume per well: 100 µL

References

1. Katayama, M. et al. (1994) Int. J. Oncol. 5:1049-1057.

2. Pittard, A. J. et al. (1996) Br. J. Anaesthesia 76:629-631.

3. Katayama, M. et al. (1994) Br. J. Cancer 69:580-585.

4. Klucky, B. et al. (2007) Cancer Res. 67:8198-8206.

5. Nishida, M. et al. (2003) APMIS 111:1067-1074.

6. Karayiannakis, A.J. et al. (2002) Surg. Endoscopy 11:1551-1554.

7. Nakayama, S. et al. (2001) Int. J. Cancer 93:667-673.




 
 
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