E-cadherin, also known as uvomorulin, cell-CAM 120/80 or L-CAM, is a calcium-dependent cell adhesion molecule found on epithelial cells in a variety of embryonic and adult tissues. Research studies indicate that unstable or reduced expression of E-cadherin correlates with cancer progression and specifically in cases of lung, breast and hepatocellular carcinoma and gastric and prostate tumors. A soluble 80 kDa form of E-cadherin, presumed to be a degradation product of the intact 120 kDa form, has been reported as elevated in the biological fluids of some cancer patients when compared against healthy individuals.
The Human E-cadherin EIA Kit is a 96-well in vitro enzyme assay intended for the quantitative determination of soluble human E-cadherin in serum, urine, cell culture supernatant and other biological fluid. It is a solid phase E-cadherin (CDH1) ELISA that utilizes two mouse monoclonal E-cadherin antibodies, one of which is coated onto the plate, and the other of which is peroxidase-labeled. This assay design allows for detection of soluble human E-cadherin via the following two-step incubation method: In the first step, biological samples are incubated in the antibody-coated microtiter plate, while in the second step, the plate is washed and incubated with the peroxidase-labeled E-cadherin antibody. A substrate (H2O2, TMBZ) is added and the reaction between the peroxidase and the substrate results in the development of color. Total soluble E-cadherin is determined by absorbance measurement via an ELISA plate reader. Accurate soluble E-cadherin sample concentration can be determined by comparing its specific absorbance with the absorbance obtained on a standard curve.