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Products >  Cell_Biology >  Cell_Adhesion_and_Extracellular_Matrix >  Antibodies_D_N >  Occludin_Rat_Monoclonal

Anti-Rat Occludin (1A8) Mouse IgM MoAb

Occludin is an integral membrane protein, encoded by the OCLN gene, that is located at tight junctions. Tight junctions act as a physical barrier to prevent solutes and water from passing freely through the paracellular space. Occludin is also known as BLCPMG. It is known to interact with several cytoplasmic proteins via its C terminus, while its extracellular loops are thought to be involved in the regulation of paracellular permeability and cell adhesion. When occludin is expressed in cells that lack tight junctions, it is able to induce cell adhesion. Mutations in the OCLN gene are associated with an autosomal recessive neurologic disorder known as band-like calcification with simplified gyration and polymicrogyria (BLC-PMG).

This product is an affinity-purified IgG antibody that recognizes rat occludin protein. The antibody was raised in mouse using recombinant occludin and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of rat, human, and dog occludin protein.

At-A-Glance Documents Images & Data

Features

  • Cat.#: 10345A, 10345B
  • Target Name: Rat occludin protein
  • Antigen: Rat occludin recombinant protein
  • Specificity: Cross-reacts with human and dog occludin
  • Application: WB, IHC
  • Target Entrez Gene ID: 83497

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 0.1–0.5 µg/mL
  • Immunohistochemical techniques: 0.5 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O (final concentration 50 µg/mL).

Alternative names

OCLN, BLCPMG




 
 
Products
Cat. # Product Package Size Price # of Units Select
10345A Anti-Rat Occludin (1A8) Mouse IgM MoAb 5 ug $113.00 *
10345B Anti-Rat Occludin (1A8) Mouse IgM MoAb 50 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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