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Products >  Cell_Biology >  Cell_Adhesion_and_Extracellular_Matrix >  Antibodies_A_C >  Claudin-5_Mouse_Rat_Antibody

Anti-Mouse Claudin-5 (C) Rabbit IgG Antibody

Claudins are a family of proteins that control the flow of molecules in the intercellular space between epithelial cells. They are the most important components of tight junctions--a form of cell-to-cell adhesion that forms continuous seals around cells. Tight junctions act as a physical barrier to prevent solutes and water from passing freely through the paracellular space. Claudins have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Over 24 members of the claudin family have been identified in humans. In mouse, claudin-1, -2, -6, -7, and -15 are expressed at high levels in liver and kidney, while claudin-5 is expressed in vascular endothelial cells. Mutations in the human CLDN5 gene are associated with velocardiofacial syndrome. Knockout mice lacking the CLDN5 gene had a lethal disruption of the blood-brain barrier. Claudin-5 is expressed at high levels in normal lung endothelium and plays a regulatory role in acrolein-induced acute lung injury. Claudin-5 is also known as MBEC1, BEC1, CPETRL1, transmembrane protein deleted in velocardiofacial syndrome (Tmvcf), AWAL, and AI854493.

This product is an affinity-purified IgG antibody that recognizes mouse claudin-5 protein. The antibody was raised in rabbit using a synthetic peptide, and can be used for Western blot (WB) detection, immunohistochemical (IHC) detection, and immunocytochemical (ICC) detection of mouse claudin-5 protein.

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Features

  • Cat.#: 18855A
  • Target Name: Mouse claudin-5 protein
  • Antigen: Mouse claudin-5 C-terminal synthetic peptide
  • Application: WB, IHC, ICC
  • Specificity: Reacts with claudin-5; does not cross-react with claudins 1-4, or 6-8
  • Target Entrez Gene ID: 12741

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 2 µg/mL
  • Immunohistochemical/immunocytochemical techniques: 5 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O (final concentration 100 µg/mL).

Alternative names

MBEC1, BEC1, CPETRL1, transmembrane protein deleted in velocardiofacial syndrome (Tmvcf), AWAL, AI854493




 
 
Products
Offers Cat. # Product Contents Size Price Units Select
18855A Anti-Mouse Claudin-5 (C) Rabbit IgG Affinity Purify 100 ug $499.00
 

 

 
 

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