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Products >  Cell_Biology >  Cell_Adhesion_and_Extracellular_Matrix >  Antibodies >  Fibronectin_Human_Monoclonal

Fibronectin (Human), Monoclonal

These monoclonal antibody clones were raised against purified human plasma fibronectin. Each recognizes discrete locations of the protein molecule (see Figure). For generic use such as Western blots or histology, clone FN30-8 works best and is also effective for adhesion blockade studies. Clones FN8-12 and FNH3-8 are unique in that they can be used for studies related to fibrin, collagen, and heparin interactions, respectively.
At-A-Glance Documents

Applications

  • Western blotting analysis under non-reducing and non-heating conditions
  • Clones FN12-8 and FN30-8 can also be used under reducing condition
  • Histology on frozen tissue section
  • Clones FN8-12, FN12-8, FN9-1, and FN30-8: Paraffin embedded tissue sections
  • Clones FN12-8 and FN30-8 : Adhesion blockade studies against fibronectin

Source

CloneCat.#ImmunogenImmunized hostSubclassAntibody titer
FN12-8M002human plasma fibronectinBALB/c mouseIgG11:10,000
FN9-1M009human plasma fibronectinBALB/c mouseIgG11:10,000
FN30-8M010human plasma fibronectinBALB/c mouseIgG11:10,000
FN1-1M013human plasma fibronectinBALB/c mouseIgG11:10,000
FNH3-8M115human plasma fibronectinBALB/c mouseIgG11:10,000

  

Storage

Lyophilized antibody is stable at 4°C for 2 years. The reconstituted solution can be stored at 4°C for 6 months, and at –20°C for 1 year.

Notes

For research use only. Not for use in diagnostic or therapeutic procedures.

Specificity

CloneCat.#Domain specificityReact withNot react with
FN12-8M002Cellhuman, bovineporcine, rabbit, rat
FN9-1M009First Fibrin-Heparinhuman, bovine, porcine, rabbitrat
FN30-8M010Cellhumanbovine, porcine, rabbit, rat
FN1-1M013C-terminal S-S bondhuman, bovine, porcine, rabbitrat (weak)
FNH3-8M115Type III (H12) in third Heparinhuman, rabbitbovine, porcine, rat

Form

Lyophilized

Reconstitution

Dissolve the lyophilized antibody in 50 µl of distilled water (final concentration: 2.0 mg/ml).

References

  1. Katayama, M. et al. (1989) Exp. Cell. Res., 185, 229-236.


 
 
Products
Cat. # Product Package Size Price
M002 Monoclonal Anti-Human Fibronectin (Clone FN12-8) 0.4 mg $309.00
M010 Monoclonal Anti-Human Fibronectin (Clone FN30-8) 0.4 mg $309.00
M115 Monoclonal Anti-Human Fibronectin (Heparin-binding domain) (Clone FNH3-8) 0.4 mg $309.00
 

 


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