Applications

• Studies on domain structures of calpastatin, or Ca²⁺-dependent enzymes by measurement of calpastatin in cell cultures supernatant
• Western blotting analysis under reducing and non-reducing conditions

Source

Storage

4°C
The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or at 4°C for 6 months.

Notes

For research use only. Not for use in diagnostic or therapeutic procedures.

Specificity

Clone specifically reacts with human calpastatin.

Form

Lyophilized

Reconstitution

Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL).

References

1. Asada, K., Ishino, Y., Shimada, M., Shimojo, T., Endo, M., Kimizuka, F., Kato, I., Maki, M., Hatanaka, M. and Murachi, T. (1989) J. Enzyme Inhib. 3, 49.
2. Towbin, H., Staehelin, T. and Gordon, J. (1979) Proc. Natl. Acad. Sci. USA 76, 4350.
3. Engvall, E. and Perlmann, P. (1972) J. Immunol. 109, 129.
4. Uemori, T. et al. (1990) Biochem. Biophys. Res. Comm. 166, 1485-1493.
5. Yokota, H. et al. (1991) Mol. Cell. Probes 5, 261-269.