Takara
login | register | order now | view cart  
Products
Cell Biology
Allergy and Immunology
Apoptosis Kits and Reagents
Bone Research
Cell Adhesion and Extracellular Matrix
    Antibodies
    EIA Kits
    Reagents
Cell Proliferation and Viability
Miscellaneous
Signal Transduction
Resources


Products >  Cell_Biology >  Cell_Adhesion_and_Extracellular_Matrix >  Antibodies >  Cadherin

Human Anti-Cadherin Antibody

Cadherins are 723-747 amino acid transmembrane glycoproteins that function as Ca2+-dependent adhesion receptors and are responsible for tight junctions1. The cadherin family has a number of members including E- (epithelial), P- (placental) and N- (neural) cadherin, all of which share a common basic structure (see below) but have distinct tissue distributions. The specific binding properties of each cadherin member appear to control selective cell-to-cell adhesion. Regulated expression of the members during development implicates their involvement in morphogenesis. Recent findings also suggest a relationship between cadherin expression and tumor invasion and metastasis1, 2.

Human anti-cadherin antibody clones are constructed by fusing immunized host splenocytes with P3-X63-Ag8 myelomas. Clones HECD-1, SHE78-7 and NCC-CAD-299 are purified from ascitic fluid. Other clones are purified from serum-free culture supernatant.

 
At-A-Glance Documents Images & Data Resources

Features

  • HECD-1/SHE78-7: for paraffin-embedded tissue
  • ECCD-1: for inhibition assays
  • Polyclonal Anti-N-cadherin: for paraffin-embedded tissue

Applications

CloneCat.#
E-cadherin
HECD-1M106Immunohistochemistry of paraffin-embedded and frozen tissue sections
Western blot analysis using non-reducing and reducing conditions
e-cadherin dependent cell-cell contact inhibition assay (100 µg/ml)
SHE78-7M126Immunohistochemistry of paraffin-embedded and frozen tissue sections
Western blot analysis using non-reducing and reducing conditions
E-cadherin dependent cell-cell contact inhibition assay (1 µg/ml)
ECCD-1M107E-cadherin dependent cell-cell contact inhibition assay (200 µg/ml)
ECCD-2M108Immunohistochemistry of frozen tissue sections
Western blot analysis using non-reducing and reducing conditions
P-cadherin
NCC-CAD-299M127Immunohistochemistry of frozen tissue sections
Western blot analysis using non-reducing and non-heating conditions
P-cadherin dependent cell-cell contact inhibition assay (100 µg/ml)
PCD-1M109Immunohistochemistry of frozen tissue sections
Western blot analysis using non-reducing and reducing conditions
P-cadherin dependent cell-cell contact inhibition assay (60 µg/ml)
N-cadherin
NCD-2M110Immunohistochemistry of frozen tissue sections
Western blot analysis using non-reducing and reducing conditions
N-cadherin dependent cell-cell contact inhibition assay (100 µg/ml)
PolyclonalM142Immunohistochemistry of paraffin-embedded and frozen tissue sections
Western blot analysis using non-reducing and non-heating conditions

Storage

Lyophilized antibody is stable at 4°C for up to 2 years. The reconstituted solution can be stored at 4°C for 6 months and at –20°C for 1 year.

Notes

For research use only. Not for diagnostic or therapeutic use.

Specificity

CloneCat.#Reacts withDoes not react with
E-cadherin
HECD-1M106human, guinea pigbovine, dog, rat, chicken
SHE78-7M126humanrat
ECCD-1M107mousehuman, bovine, chicken, dog
ECCD-2M108mouse, human, dogbovine, chicken
P-cadherin
NCC-CAD-299M127human
PCD-1M109mousehuman, chicken
N-cadherin
NCD-2M110chicken, xenopushuman, mouse
PolyclonalM142human, bovine, mouse, chickenhuman E, P-cadherin

Form

Lyophilized

Reconstitution

Monoclonal antibody (M106, M126, M107, M108, M109, M127, M110): Dissolve in 50 µL of distilled water (final conc.: 2.0 mg/mL, no preservative)

Polyclonal antibody (M142): Dissolve in 200 µL of distilled water (final conc.: 2.0 mg/mL, containing 0.1% sodium azide)

References

  1. Takeichi, M. (1991) Science 251:1451-1455.
  2. Bussemakers, M. J. G. et al. (1992) Cancer Res. 52:2916-2922.
  3. Nose, A. et al. (1990) Cell 61:147-155.
  4. Shimoyama, Y. et al. (1989) Cancer Res. 49:2128-2133.
  5. Yoshida-Noro, C. et al. (1984) Dev. Biol. 101:19-27.
  6. Shirayoshi, Y. et al. (1986) Cell Struct. Funct. 11:245-252.
  7. Nose, A. & Takeichi, M. (1986) J. Cell Biol. 103:2649-2658.
  8. Hatta, K. & Takeichi, M. (1986) Nature 320:447-449.
  9. Takeichi, M. (1988) Development 102:639-655.
  10. Hatta, K. et al. (1987) Devel. Biol. 120:215-227.
  11. Takeichi, M. (1990) Annu. Rev. Biochem. 59:237-252.
  12. Shimamura, K. et al. (1992) Dev. Biol. 152:242-254.
  13. Shimamura, K. & Takeichi, M. (1992) Development 116:1011-1019.
  14. Katayama, M. et al. (1994) Int. J. Oncol. 5:1049-1057.
  15. Shimoyama, Y. et al. (1989) Cancer Res. 49:2128-2133.



 
 
Products
Cat. # Product Package Size Price # of Units Select
M106 Monoclonal Anti-Human E-cadherin (Clone HECD-1) 0.1 mg $304.00
M126 Monoclonal Anti-Human E-cadherin (Clone SHE78-7) 0.1 mg $304.00
M127 Monoclonal Anti-Human P-cadherin (Clone NCC-CAD-299) 0.1 mg $304.00
M110 Monoclonal Anti-chicken N-Cadherin (Clone NCD-2) 0.1 mg $304.00
M107 Monoclonal Anti-mouse E-cadherin (Clone ECCD-1) 0.1 mg $304.00
M108 Monoclonal Anti-mouse E-cadherin (Clone ECCD-2) 0.1 mg $304.00
M109 Monoclonal Anti-mouse P-cadherin (Clone PCD-1 ) 0.1 mg $304.00
M142 Polyclonal Anti-N-cadherin 0.4 mg $255.00
 

 


Clontech is a Takara Bio Company © 2013 Privacy Policy | Terms & Conditions | TRUSTe