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Products >  Cell_Biology >  Cell_Adhesion_and_Extracellular_Matrix >  Antibodies >  Cadherin

Cadherin

Cadherin monoclonal antibody clones originating from relevant hybridoma cells are constructed by fusion between splenocytes of immunized hosts and P3-X63-Ag8 myelomas. HECD-1, SHE78-7, and NCC-CAD-299 are purified from ascitic fluids. The other clones are purified from serum-free culture supernatant.

Cadherins are transmembrane glycoproteins of 723-747 amino acids which function as Ca2+-dependent adhesion receptors, and are responsible for tight intercellular connections1. The cadherin family has a number of members including E- (epithelial), P- (placental), and N- (neural) cadherin, which share a common basic structure (see below) but show distinct tissue distributions. The specific binding properties of each member appear to control selective cell-to-cell adhesion. Regulated expression of the members during development implicates involvement in morphogenesis. Recent findings also suggest relationships with tumor invasion and metastasis1, 2.
At-A-Glance Documents Images & Data Resources

Features

  • HECD-1/SHE78-7: for paraffin-embedded tissue
  • ECCD-1: for inhibition assay
  • Polyclonal Anti-N-cadherin/Anti-P, E, N-cadherin: for paraffin-embedded tissue

Applications

CloneCat.#
E-cadherin
HECD-1M106Histology on paraffin embedded tissue and frozen tissue sections
Western blotting analysis under non-reducing and reducing conditions
Inhibition assay of E-cadherin dependent cell-cell contact (100 µg/ml)
SHE78-7M126Histology on paraffin embedded tissue and frozen tissue sections
Western blotting analysis under non-reducing and reducing conditions
Inhibition assay of E-cadherin dependent cell-cell contact (1 µg/ml)
ECCD-1M107Inhibition assay of E-cadherin dependent cell-cell contact (200 µg/ml)
ECCD-2M108Histology on frozen tissue sections
Western blotting analysis under non-reducing and reducing conditions
P-cadherin
NCC-CAD-299M127Histology on frozen tissue sections
Western blotting analysis under non-reducing and non-heating conditions
Inhibition assay of P-cadherin dependent cell-cell contact (100 µg/ml)
PCD-1M109Histology on frozen tissue sections
Western blotting analysis under non-reducing and reducing conditions
Inhibition assay of P-cadherin dependent cell-cell contact (60 µg/ml)
N-cadherin
Ncad 1-1-3M180Histology on paraffin embedded tissue and frozen tissue sections
Western blotting analysis under non-reducing and non-heating conditions
Immunoprecipitation
NCD-2M110Histology on frozen tissue sections
Western blotting analysis under non-reducing and reducing conditions
Inhibition assay of N-cadherin dependent cell-cell contact (100 µg/ml)
PolyclonalM142Histology on paraffin embedded tissue and frozen tissue sections
Western blotting analysis under non-reducing and non-heating conditions
P,E,N-cadherin
PolyclonalM143Histology on paraffin embedded tissue and frozen tissue sections
Western blotting analysis under non-reducing and reducing conditions

Storage

Lyophilized antibody is stable at 4°C for 2 years. The reconstituted solution can be stored at 4°C for 6 months, and at –20°C for 1 year.

Notes

For research use only. Not for use in diagnostic or therapeutic procedures.

Specificity

CloneCat.#Reacts withDoes not react with
E-cadherin
HECD-1M106human, guinea pigbovine, dog, rat, chicken
SHE78-7M126humanrat
ECCD-1M107mousehuman, bovine, chicken, dog
ECCD-2M108mouse, human, dogbovine, chicken
P-cadherin
NCC-CAD-299M127human
PCD-1M109mousehuman, chicken
N-cadherin
Ncad 1-1-3M180human, rabbithuman E, P-cadherin
NCD-2M110chicken, xenopushuman, mouse
PolyclonalM142human, bovine, mouse, chickenhuman E, P-cadherin
P,E,N-cadherin
PolyclonalM143human P, E, N-cadherin
chicken N-cadherin, mouse E, P-cadherin

Form

Lyophilized.

Reconstitution

Monoclonal (M106, M126, M107, M108, M109, M127, M110). Dissolve in 50 µL of distilled water (final conc.: 2.0 mg/mL, not containing any preservative)

Polyclonal (M142, M143). Dissolve in 200 µL of distilled water (final conc.: 2.0 mg/mL, containing 0.1% sodium azide)

References

  1. Takeichi, M. (1991) Science, 251, 1451-1455.
  2. Bussemakers, M. J. G. et al. (1992) Cancer Res., 52, 2916-2922.
  3. Nose, A. et al. (1990) Cell, 61, 147-155.
  4. Shimoyama, Y. et al. (1989) Cancer Res., 49, 2128-2133.
  5. Yoshida-Noro, C., et al. (1984) Dev. Biol., 101, 19-27.
  6. Shirayoshi, Y. et al. (1986) Cell Struct. Funct., 11, 245-252.
  7. Nose, A. and Takeichi, M. (1986) J. Cell Biol., 103, 2649-2658.
  8. Hatta, K. and Takeichi, M. (1986) Nature, 320, 447-449.
  9. Takeichi, M. (1988) Development, 102, 639-655.
  10. Hatta, K. et al. (1987) Dev. Biol., 120, 215-227.
  11. Takeichi, M. (1990) Annu. Rev. Biochem., 59, 237-252.
  12. Shimamura, K. et al. (1992) Dev. Biol., 152, 242-254.
  13. Shimamura, K. and Takeichi, M. (1992) Development, 116, 1011-1019.
  14. Katayama, M. et al. (1994) Int. J. Oncol., 5, 1049-1057.
  15. Shimoyama, Y. et al. (1989) Cancer Res., 49, 2128-2133.



 
 
Products
Cat. # Product Package Size Price
M106 Monoclonal Anti-Human E-cadherin (Clone HECD-1) 0.1 mg $298.00
M126 Monoclonal Anti-Human E-cadherin (Clone SHE78-7) 0.1 mg $298.00
M127 Monoclonal Anti-Human P-cadherin (Clone NCC-CAD-299) 0.1 mg $298.00
M110 Monoclonal Anti-chicken N-Cadherin (Clone NCD-2) 0.1 mg $298.00
M107 Monoclonal Anti-mouse E-cadherin (Clone ECCD-1) 0.1 mg $298.00
M108 Monoclonal Anti-mouse E-cadherin (Clone ECCD-2) 0.1 mg $298.00
M109 Monoclonal Anti-mouse P-cadherin (Clone PCD-1 ) 0.1 mg $298.00
M142 Polyclonal Anti-N-cadherin 0.4 mg $250.00
 

 


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