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Products >  Cell_Biology >  Cancer_and_Inflammation >  ELISA_Kits_N-Z >  VEGFR-3_Fit-4_Human

Human VEGFR-3/Flt-4 ELISA Kit

The vascular endothelial growth factor (VEGF) family of cell signaling proteins regulates vasculogenesis during embryonic development and angiogenesis in adults. Members of this family include placenta growth factor (PGF), VEGF-B, VEGF-C, and VEGF-D. Vascular endothelial growth factor receptor 3 (VEGFR-3), encoded by the human FLT4 gene, is a cell-surface tyrosine kinase receptor that binds VEGF-C and VEGF-D. It is involved in adult lymphangiogenesis and maintenance of the lymphatic endothelium. Activation of VEGFR-3/FLT4 results in upregulation of VEGF-C expression, creating a positive feedback loop. VEGFR-3/FLT4 activates several downstream signaling pathways through the action of MAPK1/ERK2, MAPK3/ERK1, MAPK8, c-Jun, and AKT1. Mutations in the FLT4 gene are associated with hereditary lymphedema type IA. VEGFR-3/FLT4 is also known as fms-related tyrosine kinase 4, PCL, LMPH1A, and FLT41.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human VEGFR-3/FLT4 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human VEGFR-3/FLT4 protein in serum, EDTA plasma, and cell culture supernatants.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring human VEGFR-3/FLT4 protein
  • Sensitivity: 0.004 ng/mL

Applications

  • Measuring human VEGFR-3/FLT4 protein in serum, EDTA plasma, and cell culture supernatants

Components

Precoated plate: Anti-Human Flt-4 (17A1) Mouse IgG MoAb Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human Flt-4 (32A1) Mouse IgG
MoAb Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Human Flt-40.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 0.03–2.0 ng/mL
  • Assay sensitivity: 0.004 ng/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Shows ≤0.1% cross-reactivity with human c-Kit, KDR, Tie-2, PDGFR-alpha, and PDGFR-beta
  • Test specimen type: Serum, EDTA plasma, and cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27779A Human VEGFR-3/Flt-4 Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 



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