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Products >  Cell_Biology >  Cancer_and_Inflammation >  ELISA_Kits_N-Z >  VEGF-C_Human

Human VEGF-C ELISA Kit

The vascular endothelial growth factor (VEGF) family of cell signaling proteins promotes embryonic development of the circulatory system (vasculogenesis), and growth of new blood vessels from existing vasculature (angiogenesis). Members of this family include placenta growth factor (PGF), VEGF-B, VEGF-C, and VEGF-D. VEGF-C undergoes complex post-translational processing, generating multiple isoforms that bind to and activate VEGFR-2 and VEGFR-3 receptors. Only the fully processed form can bind the VEGFR-2 receptor. VEGF-C regulates increased vascular permeability, and endothelial cell growth and migration. It may also control angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and maintain the differentiated lymphatic endothelium in adults. VEGF-C is also known as vascular endothelial growth factor-related protein (VRP), FLT4 ligand DHM, and Flt4 ligand (Flt4-L).

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human VEGF-C protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human VEGF-C protein in serum, EDTA plasma, heparin plasma, and cell culture supernatants.

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Features

  • Capable of measuring recombinant or native human VEGF-C protein
  • Sensitivity: 11.72 pg/mL

Applications

  • Measuring human VEGF-C protein in serum, EDTA plasma, heparin plasma, and cell culture supernatants

Components

Precoated plate: Anti-Human VEGF-C(13C1) Mouse IgG MoAb
Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human VEGF-C
(408) Rabbit IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Human VEGF-C0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 93.75–6000 pg/mL
  • Assay sensitivity: 11.72 pg/mL
  • Assay duration: Approximately 3–3.5 hours
  • Test specimen type: Serum, EDTA plasma, heparin plasma, and cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Contents Size Price Units Select
27756A Human VEGF-C Assay Kit-IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

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