Takara
login | register | order now | view cart | feedback
Products
Cell Biology


Products >  Cell_Biology >  Cancer_and_Inflammation >  ELISA_Kits_N-Z >  VEGF

VEGF ELISA Kits

Vascular endothelial growth factor (VEGF) is a cell signaling protein that promotes embryonic development of the circulatory system (vasculogenesis), and growth of new blood vessels from existing vasculature (angiogenesis). It is also known as VEGF-A, vascular permeability factor, and MVCD1. Human VEGF is a 38 kDa homodimeric protein that consists of two 165 amino-acid polypeptide chains. VEGF is expressed in many human tumor cells, and the consequent increased vascularization enables tumors to grow rapidly. Other members of the VEGF family include placenta growth factor (PGF), VEGF-B, VEGF-C, and VEGF-D.

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to human, mouse, or rat VEGF protein; one is precoated on the ELISA plate, and the other is HRP-conjugated. These kits can be used to measure soluble human, mouse, or rat VEGF protein in EDTA plasma or cell culture supernatants.

At-A-Glance Documents Images & Data Resources

Features

  • Capable of measuring human, mouse, or rat VEGF
  • Sensitivity of 5.44 pg/mL (Cat.# 27101); 10.98 pg/mL (Cat.# 27102); 1.01 pg/mL (Cat.# 27171)

Applications

  • Measuring human, mouse or rat VEGF protein in EDTA plasma or cell culture supernatants

Components

Precoated plate: Anti-VEGF IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-VEGF-1 Rabbit
IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant VEGF0.5 mL x 2
EIA buffer:30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance:

  • Range of assay: 31.25–2000 pg/mL (Cat.# 27101); 62.5–4000 pg/mL (Cat.# 27102); 15.63–1000 pg/mL (Cat.# 27171)
  • Assay sensitivity: 5.44 pg/mL (Cat.# 27101); 10.98 pg/mL (Cat.# 27102); 1.01 pg/mL (Cat.# 27171)
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Rat VEGF-164 (Cat.# 27101); mouse VEGF-164 (Cat.# 27102); human VEGF-165, VEGF-121 (Cat.# 27171)
  • Species cross-reactivity: Each kit shows ≤0.1% cross-reactivity with the other species VEGF
  • Test specimen type: EDTA plasma or cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27101A Rat VEGF Assay Kit - IBL 96-Well $606.00 *
27102A Mouse VEGF Assay Kit - IBL 96-Well $606.00 *
27171A Human VEGF Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions