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Products >  Cell_Biology >  Cancer_and_Inflammation >  ELISA_Kits_N-Z >  Thioredoxin_Human

Thioredoxin Assay

The Thioredoxin Assay is a solid-phase sandwich ELISA that uses two highly specific antibodies to human thioredoxin protein; one antibody is precoated onto the thioredoxin ELISA plate and the other antibody is HRP-conjugated. The thioredoxin assay can be used to measure soluble human thioredoxin protein in serum, EDTA-plasma, urine or cell culture supernatant.

Thioredoxins are a class of redox proteins found in all organisms. In humans, two isoforms of thioredoxin are encoded by the TXN gene. Thioredoxin is also known as surface-associated sulphydryl protein (SASP), ATL-derived factor (ADF), TRX, TRDX and TRX1. Thioredoxins are characterized by the presence of two neighboring cysteines arranged in a CXXC motif at the active site. Thioredoxins are maintained in the reduced state by the flavoenzyme thioredoxin reductase through an NADPH-dependent reaction. Thioredoxins act as electron donors to peroxidases and ribonucleotide reductase. Several transcription factors, such as NF-kappa B and AP1, also require thioredoxin-mediated reduction to exert DNA-binding activity. Thioredoxin knockout mammal embryos die in utero. Numerous other functions for thioredoxin have been described, including oxidative stress protection and control of growth and apoptosis.


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Features

Cat.# 27417A

  • Capable of measuring human thioredoxin protein
  • Sensitivity: 0.43 ng/mL

Applications

  • Measurement of human thioredoxin protein in serum, EDTA-plasma, urine or cell culture supernatant

Components

Precoated plate: Anti-Human Thioredoxin (5t1) Mouse IgG MoAb,
affinity-purified
96 Well x 1
Labeled target: HRP-conjugated Anti-Human Thioredoxin (N) Rabbit
IgG Fab, affinity-purified
0.4 mL x 1
Standard: Recombinant Human Thioredoxin1.0 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 3.91–250 ng/mL
  • Assay sensitivity: 0.43 ng/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Human thioredoxin
  • Test specimen type: Serum, EDTA-plasma, urine or cell culture supernatant
  • Sample volume per well: 100 µL

 
Products
Offers Cat. # Product Contents Size Price Units Select
27417A Human Thioredoxin Assay Kit - IBL 96-Well $606.00 *
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