Takara
login | register | order now | view cart | feedback
Products
Cell Biology


Products >  Cell_Biology >  Cancer_and_Inflammation >  ELISA_Kits_N-Z >  Tenascin-C_Large_Human

Tenascin-C ELISA

The Tenascin-C ELISA kit is a solid-phase sandwich ELISA that uses two highly specific antibodies to nonspliced high molecular weight variants of tenascin-C protein; one of these antibodies is precoated onto the Tenascin-C ELISA plate and the other is HRP-conjugated. The Tenascin-C ELISA kits can be used to measure soluble human (Cat.# 27751) or human/mouse/rat (Cat.# 27767) tenascin-C "large" proteins in serum, plasma or cell culture supernatants.

Tenascin-C (also known as cytotactin, hexabrachion, HXB, J1, neuronectin, glioma-associated-extracellular matrix antigen and GMEM) is a multifunctional extracellular matrix glycoprotein. It consists of multiple subunits with a molecular weight ranging from 210-400 kDa. The subunits contain several repeated motifs, including those with similarity to epidermal growth factor (EGF), fibronectin type III (FNIII), and the globular domain of the beta and gamma chains of fibrinogen. In the embryonic stage, tenascin-C is expressed in migrating cells like the neural crest and in developing tendons, bone and cartilage. In adults, studies have suggested that tenascin-C is upregulated during pathological processes such as oncogenesis and arthritis as well as liver and lung fibrosis.

At-A-Glance Documents Images & Data Resources

Features

  • Capable of measuring nonspliced high molecular weight variants of tenascin-C
  • Sensitivity of 0.01 ng/mL (Cat.# 27751); 44 pg/mL (Cat.# 27767)

Applications

  • Measuring tenascin-C "large" proteins in serum, plasma, or cell culture supernatants

Components

Precoated plate: Anti-Tenascin-C Mouse IgG MoAb Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human Tenascin-C (4F10TT)
Mouse IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Purified Human Tenascin-C0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 0.38–24 ng/mL (Cat.# 27751); 0.20–12.5 ng/mL (Cat.# 27767)
  • Assay sensitivity: 0.01 ng/mL (Cat.# 27751); 44 pg/mL (Cat.# 27767)
  • Assay duration: Approximately 3-3.5 hours
  • Specificity: High molecular weight variants of human tenascin-C (Cat # 27751); human, mouse or rat tenascin-C (Cat.# 27767)
  • Species cross-reactivity: n/a (Cat.# 27751); human, mouse or rat tenascin-C (Cat.# 27767)
  • Test specimen type: Serum, plasma or cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27767A Tenascin-C Large (FN Ⅲ-B) Assay Kit - IBL 96-Well $637.00 *
27751A Human Tenascin-C Large (FN Ⅲ-C) Assay Kit 96-Well $637.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 



Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions