The Tenascin-C ELISA kit is a solid-phase sandwich ELISA that uses two highly specific antibodies to nonspliced high molecular weight variants of tenascin-C protein; one of these antibodies is precoated onto the Tenascin-C ELISA plate and the other is HRP-conjugated. The Tenascin-C ELISA kits can be used to measure soluble human (Cat.# 27751) or human/mouse/rat (Cat.# 27767) tenascin-C "large" proteins in serum, plasma or cell culture supernatants.
Tenascin-C (also known as cytotactin, hexabrachion, HXB, J1, neuronectin, glioma-associated-extracellular matrix antigen and GMEM) is a multifunctional extracellular matrix glycoprotein. It consists of multiple subunits with a molecular weight ranging from 210-400 kDa. The subunits contain several repeated motifs, including those with similarity to epidermal growth factor (EGF), fibronectin type III (FNIII), and the globular domain of the beta and gamma chains of fibrinogen. In the embryonic stage, tenascin-C is expressed in migrating cells like the neural crest and in developing tendons, bone and cartilage. In adults, studies have suggested that tenascin-C is upregulated during pathological processes such as oncogenesis and arthritis as well as liver and lung fibrosis.