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Products >  Cell_Biology >  Cancer_and_Inflammation >  ELISA_Kits_N-Z >  SCF_Human

Human SCF ELISA Kit

Stem cell factor (SCF) and its receptor c-Kit can exist both as a transmembrane protein and a soluble protein. SCF is also known as Kit ligand (KL-1, Kitl), steel factor (SF), mast cell growth factor (MGF), familial progressive hyperpigmentation 2 (FPH2), and SHEP7. It plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. Functional SCF and c-Kit are critical to the development and survival of hematopoietic stem cells. Interaction of SCF with c-Kit, a tyrosine kinase, results in increased autophosphorylation activity.

This kit is a solid-phase sandwich ELISAs using two antibodies that are highly specific to human SCF protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure soluble human SCF protein in cell culture supernatant.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring human SCF
  • Sensitivity: 7.14 pg/mL

Applications

  • Measuring human SCF in cell culture supernatant

Components

Precoated plate: Anti-Human SCF (K089) Rabbit IgG Affinity-Purified96 Well x 1
Labeled target: HRP conjugated Anti-Human SCF (K151) Rabbit IgG Fab
Affinity-Purified
0.4 mL x 1
Standard: Recombinant Human SCF0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 50–3,200 pg/mL
  • Assay sensitivity: 7.14 pg/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Human SCF
  • Species cross-reactivity: n/a
  • Test specimen type: Cell culture supernatant
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27141A Human SCF Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 



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